Datta H K, Zaidi M, Wimalawansa S J, Ghatei M A, Beacham J L, Bloom S R, MacIntyre I
Department of Chemical Pathology and Medicine, Royal Postgraduate Medical School, London, UK.
Biochem Biophys Res Commun. 1989 Jul 31;162(2):876-81. doi: 10.1016/0006-291x(89)92391-7.
Amylin is a new member of the calcitonin/CGRP family: it is a 37 amino acid polypeptide which was recently isolated from amyloid deposits in pancreatic islets obtained from type II diabetics. In the present study we investigated the effect of amylin and amylin-amide on calcium metabolism in the rat and rabbit. Two main methods were used: in vivo hypocalcaemic activity was assessed by measuring plasma calcium levels after injection of the peptide in 50 g rats; and in vitro resorption of cortical bone by disaggregated rat osteoclasts was quantified by scanning electron microscopy together with image analysis. We demonstrate that amylin and amylin-amide have calcitonin-like effects: both are powerful inhibitors of osteoclastic resorption and as a consequence lower plasma calcium in both rats and rabbits. We speculate that the peptide may exert systemic or local regulatory effects on bone cells.
胰淀素是降钙素/CGRP家族的新成员：它是一种由37个氨基酸组成的多肽，最近从II型糖尿病患者的胰岛淀粉样沉积物中分离出来。在本研究中，我们研究了胰淀素和胰淀素酰胺对大鼠和兔子钙代谢的影响。采用了两种主要方法：通过测量50g大鼠注射该肽后血浆钙水平来评估体内降钙活性；通过扫描电子显微镜结合图像分析对大鼠破骨细胞解离后的皮质骨体外吸收进行定量。我们证明胰淀素和胰淀素酰胺具有类似降钙素的作用：两者都是破骨细胞吸收的强力抑制剂，因此会降低大鼠和兔子的血浆钙水平。我们推测该肽可能对骨细胞产生全身或局部调节作用。
