核苷酸焦磷酸酶/磷酸二酯酶 2 在斑马鱼中轴形成中的作用。
Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
机构信息
Australian Regenerative Medicine Institute, Building 75, Monash University, Australia.
Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital &Ophthalmology, the University of Melbourne, Department of Surgery, Australia.
出版信息
Sci Rep. 2016 Nov 24;6:37678. doi: 10.1038/srep37678.
Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV), through the modulation of cell migration during gastrulation in a lpar Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.
溶血磷脂酸（LPA）是一种独特的生物活性溶血磷脂，通过与其特定的受体结合，在各种细胞类型和生物体中发挥多种效应。LPA 主要由细胞外核苷酸焦磷酸酶/磷酸二酯酶 2/自分泌运动因子（enpp2）合成。改变的 LPA 信号与胚胎异常有关，提示 LPA 在发育过程中具有关键作用。然而，LPA 信号在早期胚胎发生中的作用尚未得到很好的确定。我们证明，早期斑马鱼胚胎中的 enpp2/LPA 信号通过调节原肠胚形成过程中的细胞迁移，导致轴和中线形成改变，左右（L-R）模式缺陷，Kupffer 囊（KV）的纤毛发生，这是一种依赖于 lpar Rho/ROCK 的方式。总的来说，这项研究表明 enpp2/LPA 信号在早期胚胎发生中具有重要作用。
Zotero 插件