空肠弯曲菌噬菌体的分离、纯化及增殖方法
Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
作者信息
Gencay Yilmaz Emre, Birk Tina, Sørensen Martine Camilla Holst, Brøndsted Lone
机构信息
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark.
National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860, Søborg, Denmark.
出版信息
Methods Mol Biol. 2017;1512:19-28. doi: 10.1007/978-1-4939-6536-6_3.
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
在此，我们描述了从预期含有大量噬菌体的样本（如鸡粪）中分离、纯化和增殖空肠弯曲菌噬菌体的方法。总体步骤如下：(1) 从样本材料中释放噬菌体；(2) 使用点滴试验观察空肠弯曲菌菌苔上的噬菌斑形成单位；(3) 分离单个噬菌斑；(4) 连续纯化程序；以及(5) 从平板裂解物中增殖纯化的噬菌体以制备原种。
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