State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Food Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture and Rural Affairs (Shanghai), Shanghai Center of Agricultural Products Quality Safety, Shanghai 201708, China.
Viruses. 2024 Sep 11;16(9):1450. doi: 10.3390/v16091450.
pv. (Xoo) is a significant bacterial pathogen responsible for outbreaks of bacterial leaf blight in rice, posing a major threat to rice cultivation worldwide. Effective management of this pathogen is crucial for ensuring rice yield and food security. In this study, we identified and characterized a novel Xoo phage, ZP3, isolated from diseased rice leaves in Zhejiang, China, which may offer new insights into biocontrol strategies against Xoo and contribute to the development of innovative approaches to combat bacterial leaf blight. Transmission electron microscopy indicated that ZP3 had a short, non-contractile tail. Genome sequencing and bioinformatic analysis showed that ZP3 had a double-stranded DNA genome with a length of 44,713 bp, a G + C content of 52.2%, and 59 predicted genes, which was similar to other OP1-type Xoo phages belonging to the genus . ZP3's endolysin LysZP was further studied for its bacteriolytic action, and the -terminal transmembrane domain of LysZP is suggested to be a signal-arrest-release sequence that mediates the translocation of LysZP to the periplasm. Our study contributes to the understanding of phage-Xoo interactions and suggests that phage ZP3 and its endolysin LysZP could be developed into biocontrol agents against this phytopathogen.
pv. (Xoo) 是一种重要的细菌病原体，可导致水稻细菌性叶斑病暴发，对全球水稻种植构成重大威胁。有效管理这种病原体对于确保水稻产量和粮食安全至关重要。在本研究中，我们从中国浙江发病的水稻叶片中分离到一种新型的 Xoo 噬菌体 ZP3，并对其进行了鉴定和特性分析，这可能为 Xoo 的生物防治策略提供新的见解，并有助于开发创新方法来防治细菌性叶斑病。透射电子显微镜表明 ZP3 具有短而不收缩的尾巴。基因组测序和生物信息学分析表明，ZP3 具有一个长度为 44713bp 的双链 DNA 基因组，G+C 含量为 52.2%，预测有 59 个基因，与属于 的其他 OP1 型 Xoo 噬菌体相似。进一步研究了 ZP3 的内溶素 LysZP 的溶菌作用，推测 LysZP 的 -末端跨膜结构域是一个信号-暂停-释放序列，介导 LysZP 向周质的转运。我们的研究有助于了解噬菌体-Xoo 的相互作用，并表明噬菌体 ZP3 和其内溶素 LysZP 可开发为针对这种植物病原体的生物防治剂。
