NS-I的分离与全基因组测序，NS-I是一种感染鱼类致病菌株的裂解性噬菌体。
Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of .
作者信息
Akmal Muhammad, Araki Kaho, Nishiki Issei, Yoshida Terutoyo
机构信息
Department of Applied Biological Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
出版信息
Phage (New Rochelle). 2023 Dec 14;4(4):151-158. doi: 10.1089/phage.2023.0019. eCollection 2023 Dec.
BACKGROUND
Nocardiosis, an infection caused by bacterium , contributes to severe economic losses in the aqua-cultured fish species and in Japan. Phage therapy is a potential solution for combating this infection.
MATERIALS AND METHODS
A lytic bacteriophage infecting (JCM3359) was isolated from water samples collected at various fish farms. The bacteriophage was characterized by electron microscopy, host range analysis, and genomic profiling.
RESULTS
The isolated lytic bacteriophage, NS-I (accession no. OP429095.1), had a siphovirus morphotype with a broad lytic spectrum, as it infected all the tested strains (15/15). Its genome was a 43,361 bp double-stranded DNA molecule, exhibiting a GC content of 67% with 94% similarity to bacteriophage Gustav at the genome level.
CONCLUSION
Lytic bacteriophage NS-I shows promise as a biocontrol agent for nocardiosis infection in fish farms and warrants further investigation.
背景
诺卡氏菌病是由细菌引起的一种感染，在日本的水产养殖鱼类中造成了严重的经济损失。噬菌体疗法是对抗这种感染的一种潜在解决方案。
材料与方法
从各个养鱼场采集的水样中分离出一种能感染诺卡氏菌（JCM3359）的裂解性噬菌体。通过电子显微镜、宿主范围分析和基因组分析对该噬菌体进行了表征。
结果
分离出的裂解性噬菌体NS-I（登录号OP429095.1）具有长尾噬菌体形态型，裂解谱广，因为它能感染所有测试的诺卡氏菌菌株（15/15）。其基因组是一个43361 bp的双链DNA分子，GC含量为67%，在基因组水平上与诺卡氏菌噬菌体古斯塔夫有94%的相似性。
结论
裂解性噬菌体NS-I有望作为养鱼场诺卡氏菌病感染的生物防治剂，值得进一步研究。
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