研究空肠弯曲菌对宿主上皮细胞的黏附和侵袭的方法。
Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.
作者信息
Negretti Nicholas M, Konkel Michael E
机构信息
School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7520, USA.
School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Life Sciences Bldg. Room 302c, Pullman, WA, 99164-7520, USA.
出版信息
Methods Mol Biol. 2017;1512:117-127. doi: 10.1007/978-1-4939-6536-6_11.
Measuring bacterial adherence and invasion of cells in vitro has enabled researchers to dissect the interactions of Campylobacter jejuni with eukaryotic cells. Numerous C. jejuni virulence determinants and host cell factors that contribute to the process of adherence, invasion, and immune modulation have been identified utilizing in vitro adherence and invasion assays. In this chapter, we describe the evaluation of C. jejuni adherence to and invasion of HeLa cells using the gentamicin-protection assay.
在体外测量细菌对细胞的黏附和侵袭，使研究人员能够剖析空肠弯曲菌与真核细胞之间的相互作用。利用体外黏附和侵袭试验，已鉴定出许多有助于空肠弯曲菌黏附、侵袭和免疫调节过程的毒力决定因素和宿主细胞因子。在本章中，我们描述了使用庆大霉素保护试验评估空肠弯曲菌对HeLa细胞的黏附和侵袭。
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