Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Departamento de Ciências Fisiológicas, Universidade de Brasília, Brasília, Brazil.
Fungal Biol. 2020 Sep;124(9):766-780. doi: 10.1016/j.funbio.2020.05.004. Epub 2020 Jun 12.
Fungi of the genus Paracoccidioides are the etiological agents of Paracoccidioidomycosis (PCM), the most prevalent mycosis in Latin America. Paracoccidioidomycosis infection is acquired by inhalation of Paracoccidioides conidia, which have first contact with the lungs and can subsequently spread to other organs/tissues. Until now, there have been no proteomic studies focusing on this infectious particle of Paracoccidioides. In order to identify the Paracoccidioides lutzii conidia proteome, conidia were produced and purified. Proteins were characterized by use of the nanoUPLC-MS approach. The strategy allowed us to identify a total of 242 proteins in P. lutzii conidia. In the conidia proteome, proteins were classified in functional categories such as protein synthesis, energy production, metabolism, cellular defense/virulence processes, as well as other processes that can be important for conidia survival. Through this analysis, a pool of ribosomal proteins was identified, which may be important for the initial processes of dimorphic transition. In addition, molecules related to energetic and metabolic processes were identified, suggesting a possible basal metabolism during this form of resistance of the fungus. In addition, adhesins and virulence factors were identified in the P. lutzii conidia proteome. Our results demonstrate the potential role that these molecules can play during early cell-host interaction processes, as well as the way in which these molecules are involved in environmental survival during this form of propagation.
巴西副球孢子菌属的真菌是副球孢子菌病（PCM）的病因，PCM 是拉丁美洲最常见的真菌病。副球孢子菌病是通过吸入巴西副球孢子菌的分生孢子而感染的，这些分生孢子首先与肺部接触，随后可以传播到其他器官/组织。到目前为止，还没有针对巴西副球孢子菌这种感染性颗粒的蛋白质组学研究。为了鉴定巴西副球孢子菌的 lutzii 分生孢子蛋白质组，我们生产并纯化了分生孢子。使用 nanoUPLC-MS 方法对蛋白质进行了表征。该策略总共鉴定了巴西副球孢子菌 lutzii 分生孢子中的 242 种蛋白质。在分生孢子蛋白质组中，蛋白质被分类为功能类别，如蛋白质合成、能量产生、代谢、细胞防御/毒力过程以及其他可能对分生孢子生存重要的过程。通过这项分析，鉴定了一组核糖体蛋白，这可能对二态性转换的初始过程很重要。此外，还鉴定了与能量和代谢过程相关的分子，表明在这种真菌的抵抗形式下可能存在基础代谢。此外，在巴西副球孢子菌的分生孢子蛋白质组中还鉴定了黏附素和毒力因子。我们的研究结果表明，这些分子在早期细胞与宿主相互作用过程中可能发挥重要作用，以及这些分子在这种繁殖形式下参与环境生存的方式。
