油菜（甘蓝型油菜）中GA 2-氧化酶6的异位表达导致拟南芥出现矮化、开花延迟和叶绿素积累增加的现象。
Ectopic expression of GA 2-oxidase 6 from rapeseed (Brassica napus L.) causes dwarfism, late flowering and enhanced chlorophyll accumulation in Arabidopsis thaliana.
作者信息
Yan Jindong, Liao Xiaoying, He Reqing, Zhong Ming, Feng Panpan, Li Xinmei, Tang Dongying, Liu Xuanming, Zhao Xiaoying
机构信息
Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China.
出版信息
Plant Physiol Biochem. 2017 Feb;111:10-19. doi: 10.1016/j.plaphy.2016.11.008. Epub 2016 Nov 15.
Gibberellins (GAs) are endogenous hormones that play an important role in higher plant growth and development. GA2-oxidase (GA2ox) promotes catabolism and inactivation of bioactive GAs or their precursors. In this study, we identified the GA2-oxidase gene, BnGA2ox6, and found it to be highly expressed in the silique and flower. Overexpression of BnGA2ox6 in Arabidopsis resulted in GA-deficiency symptoms, including inhibited elongation of the hypocotyl and stem, delayed seed germination, and late flowering. BnGA2ox6 overexpression reduced silique growth, but had no effect on seed development. Additionally, BnGA2ox6 overexpression enhanced chlorophyll b and total chlorophyll accumulation, and downregulated mRNA expression levels of the CHL1 and RCCR genes, which are involved in the chlorophyll degradation. These findings suggest that BnGA2ox6 regulates plant hight, silique development, flowering and chlorophyll accumulation in transgenic Arabidopsis.
赤霉素（GAs）是一类内源激素，在高等植物的生长发育过程中发挥着重要作用。GA2-氧化酶（GA2ox）促进生物活性赤霉素或其前体的分解代谢及失活。在本研究中，我们鉴定出GA2-氧化酶基因BnGA2ox6，并发现其在角果和花中高表达。在拟南芥中过表达BnGA2ox6会导致赤霉素缺乏症状，包括下胚轴和茎的伸长受到抑制、种子萌发延迟以及开花推迟。BnGA2ox6过表达会减少角果生长，但对种子发育没有影响。此外，BnGA2ox6过表达增强了叶绿素b和总叶绿素的积累，并下调了参与叶绿素降解的CHL1和RCCR基因的mRNA表达水平。这些发现表明，BnGA2ox6在转基因拟南芥中调控植株高度、角果发育、开花及叶绿素积累。
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