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对（ Franch.）skeels 中赤霉素氧化酶基因的表征及相互作用蛋白筛选。

Characterization of the gibberellic oxidase gene in (Franch.) skeels and interaction protein screening.

作者信息

Zhao Lili, Xie Wenhui, Huang Lei, Long Sisi, Wang Puchang

机构信息

College of Animal Science, Guizhou University, Guiyang, China.

School of Life Sciences, Guizhou Normal University, Guiyang, China.

出版信息

Front Plant Sci. 2024 Oct 16;15:1478854. doi: 10.3389/fpls.2024.1478854. eCollection 2024.

DOI:10.3389/fpls.2024.1478854
PMID:39479549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11521860/
Abstract

Gibberellin 20-oxidases () are multifunctional enzymes involved in regulating gibberellin (GA) biosynthesis and controlling plant growth. We identified and characterized the GA20ox1 gene in a plant height mutant of , referred to as . This gene was expressed in root, stem, and leaf tissues of the adult plant height mutant, with the highest expression observed in the stem. The expression of was regulated by various exogenous hormones. Overexpression of in Arabidopsis resulted in significant elongation of hypocotyl and root length in seedlings, earlier flowering, smaller leaves, reduced leaf chlorophyll content, lighter leaf color, a significant increase in adult plant height, and other phenotypes. Additionally, transgenic plants exhibited a substantial increase in biologically active endogenous GAs (GA1, GA3, and GA4) content, indicating that overexpression of accelerates plant growth and development. Using a yeast two-hybrid (Y2H) screen, we identified two SdGA20ox1-interacting proteins: the ethylene receptor (11430582) and the (11416005) protein. These interactions suggest a potential regulatory mechanism for growth. Our findings provide new insights into the role of and its interacting proteins in regulating the growth and development of .

摘要

赤霉素20-氧化酶（）是参与调节赤霉素（GA）生物合成和控制植物生长的多功能酶。我们在一个被称为的植物高度突变体中鉴定并表征了GA20ox1基因。该基因在成年植物高度突变体的根、茎和叶组织中表达，在茎中表达量最高。的表达受多种外源激素调控。在拟南芥中过表达导致幼苗下胚轴和根长度显著伸长、开花提前、叶片变小、叶片叶绿素含量降低、叶色变浅、成年植株高度显著增加以及其他表型。此外，转基因植物中生物活性内源GA（GA1、GA3和GA4）含量大幅增加，表明过表达加速了植物的生长和发育。通过酵母双杂交（Y2H）筛选，我们鉴定出两种与SdGA20ox1相互作用的蛋白：乙烯受体（11430582）和（11416005）蛋白。这些相互作用提示了一种潜在的生长调节机制。我们的研究结果为及其相互作用蛋白在调节生长发育中的作用提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/f38fa7f4f7de/fpls-15-1478854-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/8759a404f416/fpls-15-1478854-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/40ad92df9d4c/fpls-15-1478854-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/0251788aeb4c/fpls-15-1478854-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/12b1a9fa0e66/fpls-15-1478854-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/a2e003bf88b8/fpls-15-1478854-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/6599734d39f2/fpls-15-1478854-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/a4a4707e34d5/fpls-15-1478854-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/f38fa7f4f7de/fpls-15-1478854-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/8759a404f416/fpls-15-1478854-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/40ad92df9d4c/fpls-15-1478854-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/0251788aeb4c/fpls-15-1478854-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/12b1a9fa0e66/fpls-15-1478854-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/a2e003bf88b8/fpls-15-1478854-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/6599734d39f2/fpls-15-1478854-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/a4a4707e34d5/fpls-15-1478854-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/789f/11521860/f38fa7f4f7de/fpls-15-1478854-g008.jpg

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