National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan, China.
Plant Biotechnol J. 2023 Aug;21(8):1611-1627. doi: 10.1111/pbi.14063. Epub 2023 May 8.
Plant hormones are the intrinsic factors that control plant development. The integration of different phytohormone pathways in a complex network of synergistic, antagonistic and additive interactions has been elucidated in model plants. However, the systemic level of transcriptional responses to hormone crosstalk in Brassica napus is largely unknown. Here, we present an in-depth temporal-resolution study of the transcriptomes of the seven hormones in B. napus seedlings. Differentially expressed gene analysis revealed few common target genes that co-regulated (up- and down-regulated) by seven hormones; instead, different hormones appear to regulate distinct members of protein families. We then constructed the regulatory networks between the seven hormones side by side, which allowed us to identify key genes and transcription factors that regulate the hormone crosstalk in B. napus. Using this dataset, we uncovered a novel crosstalk between gibberellin and cytokinin in which cytokinin homeostasis was mediated by RGA-related CKXs expression. Moreover, the modulation of gibberellin metabolism by the identified key transcription factors was confirmed in B. napus. Furthermore, all data were available online from http://yanglab.hzau.edu.cn/BnTIR/hormone. Our study reveals an integrated hormone crosstalk network in Brassica napus, which also provides a versatile resource for future hormone studies in plant species.
植物激素是控制植物发育的内在因素。在模式植物中，不同植物激素途径的整合已在一个协同、拮抗和相加相互作用的复杂网络中得到阐明。然而，油菜中激素相互作用的系统水平转录反应在很大程度上尚不清楚。在这里，我们对油菜幼苗中七种激素的转录组进行了深入的时间分辨率研究。差异表达基因分析显示，很少有共同的靶基因被七种激素共同调控（上调和下调）；相反，不同的激素似乎调节不同的蛋白质家族成员。然后，我们并排构建了七种激素之间的调控网络，这使我们能够识别调控油菜激素相互作用的关键基因和转录因子。利用这个数据集，我们揭示了赤霉素和细胞分裂素之间的一种新的相互作用，其中细胞分裂素的动态平衡是由 RGA 相关的 CKXs 表达介导的。此外，鉴定的关键转录因子对赤霉素代谢的调节在油菜中得到了证实。此外，所有数据均可从 http://yanglab.hzau.edu.cn/BnTIR/hormone 在线获取。我们的研究揭示了油菜中一个综合的激素相互作用网络，这也为植物物种中未来的激素研究提供了一个多功能资源。
