School of Materials Science and Engineering, Hebei University of Technology, 300130, Tianjin, China.
Institute of Energy Resources, Hebei Academy of Sciences, 050081, Shijiazhuang, Hebei Province, China.
ChemistryOpen. 2021 Mar;10(3):373-379. doi: 10.1002/open.202000336. Epub 2021 Feb 25.
Polymer-protein complexing systems have been extensively studied because of their wide application in biomedicine and industry. Here, we studied the aggregation behavior of the hydrophobically associating water-soluble polymer poly(acrylic acid-co-octadecyl methacrylate) [P(AA-co-OMA)] prepared with nonionic surfactant as an emulsifier and bovine serum albumin (BSA) in aqueous solution. We identified the optimal composite conditions of P(AA-co-OMA) and BSA aqueous solution. We measured the zeta potential, dynamic light-scattering particle size, and surface tension of P(AA-co-OMA) and BSA mixed aqueous solution. The results showed that the aggregation behavior between the polymer and BSA relied mainly on the hydrophobic interactions between the molecules. In addition, the best compounding condition was 8 wt.% of P(AA-co-OMA) content. The structure of hydrophobically associating polymer P(AA-co-OMA) and its aggregation with BSA were characterized by Fourier-transform infrared spectroscopy. The infrared spectroscopy results identified the hydrogen bonding behavior of the amino and carboxyl groups between the polymer and BSA. This behavior was also confirmed using thermogravimetric analysis and differential scanning calorimetry. The thermal decomposition temperature and melting temperature of BSA changed before and after it was combined with the polymer. We measured the morphology of the polymer BSA aggregate with 8 % polymer content by transmission electron microscopy. The binding mechanism was investigated, as well.
聚合物-蛋白质复合体系因其在生物医药和工业中的广泛应用而受到广泛研究。在这里，我们研究了以非离子表面活性剂作为乳化剂制备的疏水性水溶性聚合物聚丙烯酸-co-十八烷基甲基丙烯酸酯[P(AA-co-OMA)]与牛血清白蛋白(BSA)在水溶液中的聚集行为。我们确定了 P(AA-co-OMA)和 BSA 水溶液的最佳复合条件。我们测量了 P(AA-co-OMA)和 BSA 混合水溶液的动电位、动态光散射粒径和表面张力。结果表明，聚合物与 BSA 之间的聚集行为主要依赖于分子间的疏水相互作用。此外，最佳的复合条件是 8wt%的 P(AA-co-OMA)含量。用傅里叶变换红外光谱对疏水缔合聚合物 P(AA-co-OMA)的结构及其与 BSA 的聚集进行了表征。红外光谱结果鉴定了聚合物和 BSA 之间氨基和羧基之间的氢键行为。这一行为也通过热重分析和差示扫描量热法得到了证实。BSA 与聚合物结合前后的热分解温度和熔融温度发生了变化。我们用透射电子显微镜测量了 8%聚合物含量的聚合物 BSA 聚集体的形态。还研究了结合机制。
