氧化型谷胱甘肽和尿酸作为早期囊性纤维化肺病的生物标志物。
Oxidized glutathione and uric acid as biomarkers of early cystic fibrosis lung disease.
作者信息
Dickerhof Nina, Turner Rufus, Khalilova Irada, Fantino Emmanuelle, Sly Peter D, Kettle Anthony J
机构信息
Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.
Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.
出版信息
J Cyst Fibros. 2017 Mar;16(2):214-221. doi: 10.1016/j.jcf.2016.10.012. Epub 2016 Nov 24.
BACKGROUND
In cystic fibrosis (CF) there is an urgent need for earlier diagnosis of pulmonary infections and inflammation using blood- and urine-based biomarkers.
METHODS
Using mass spectrometry, oxidation products of glutathione and uric acid were measured in matched samples of bronchoalveolar lavage (BAL), serum and urine from 36 infants and children with CF, and related to markers of neutrophilic inflammation and infection in BAL.
RESULTS
Oxidation products of glutathione (glutathione sulfonamide, GSA) and uric acid (allantoin), were elevated in BAL of children with pulmonary infections with Pseudomonas aeruginosa (PsA) compared to those without (p<0.05) and correlated with other markers of neutrophilic inflammation. Serum GSA was significantly elevated in children with PsA infections (p<0.01). Urinary GSA correlated with pulmonary GSA (r=0.42, p<0.05) and markers of neutrophilic inflammation.
CONCLUSIONS
This proof-of-concept study demonstrates that urinary GSA but not allantoin shows promise as a non-invasive marker of neutrophilic inflammation in early CF lung disease.
背景
在囊性纤维化（CF）中，迫切需要利用基于血液和尿液的生物标志物对肺部感染和炎症进行早期诊断。
方法
采用质谱法，对36例CF婴幼儿的支气管肺泡灌洗（BAL）液、血清和尿液的配对样本中谷胱甘肽和尿酸的氧化产物进行测量，并与BAL液中嗜中性粒细胞炎症和感染的标志物相关联。
结果
与未感染铜绿假单胞菌（PsA）的儿童相比，感染PsA的儿童BAL液中谷胱甘肽（谷胱甘肽磺酰胺，GSA）和尿酸（尿囊素）的氧化产物升高（p<0.05），且与嗜中性粒细胞炎症的其他标志物相关。PsA感染儿童的血清GSA显著升高（p<0.01）。尿GSA与肺部GSA相关（r=0.42，p<0.05）以及嗜中性粒细胞炎症标志物相关。
结论
这项概念验证研究表明，尿GSA而非尿囊素有望作为CF早期肺部疾病嗜中性粒细胞炎症的非侵入性标志物。
Zotero 插件