Jung Ju Yeon, Park Ji-Hye, Oh Yu-Li, Kwon Han-Sol, Park Hyun-Chul, Park Kyung-Hwa, Kim Eun Hye, Lee Dong-Sub, Lim Si-Keun
Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea.
Leg Med (Tokyo). 2016 Nov;23:17-20. doi: 10.1016/j.legalmed.2016.09.001. Epub 2016 Sep 2.
In this study, we compared two recently released commercial Y-chromosomal short tandem repeat (Y-STR) kits: the PowerPlex Y23 System (PPY23) and Yfiler® Plus PCR amplification kit (YPlus). We performed validation studies, including sensitivity, tolerance to PCR inhibitors, and mixture analysis, and a population genetics study using 306 unrelated South Korean males. PPY23 and YPlus showed similar sensitivity, but PPY23 showed higher tolerance to humic acid than YPlus. Furthermore, the detection rate of unique minor alleles called from male/male mixtures was higher for PPY23 than for YPlus. Comparing the newly added loci, the mean values of gene diversity for PPY23 and YPlus were 0.6715 and 0.8158, respectively. The discrimination capacity in the 306 unrelated South Korean males for PPY23 was 0.9837, and that for YPlus was 0.9935. These results will inform the selection of suitable Y-STR kits based on the purpose of forensic DNA analysis.
在本研究中，我们比较了两种最近发布的商用Y染色体短串联重复序列（Y-STR）试剂盒：PowerPlex Y23系统（PPY23）和Yfiler® Plus PCR扩增试剂盒（YPlus）。我们进行了验证研究，包括灵敏度、对PCR抑制剂的耐受性和混合样本分析，并使用306名无关的韩国男性进行了群体遗传学研究。PPY23和YPlus表现出相似的灵敏度，但PPY23对腐殖酸的耐受性高于YPlus。此外，PPY23从男性/男性混合样本中检测出独特小等位基因的比率高于YPlus。比较新添加的基因座，PPY23和YPlus的基因多样性平均值分别为0.6715和0.8158。在306名无关的韩国男性中，PPY23的鉴别能力为0.9837，YPlus的鉴别能力为0.9935。这些结果将为根据法医DNA分析目的选择合适的Y-STR试剂盒提供参考。
