Calderon Silvia, Perez-Benedico David, Mesa Lazaro, Guyton Daniel, Rowold Diane J, Herrera Rene J
Department of Molecular and Human Genetics, College of Medicine, Florida International University, Miami, FL 33199, USA.
Leg Med (Tokyo). 2013 Nov;15(6):289-92. doi: 10.1016/j.legalmed.2013.07.003. Epub 2013 Aug 26.
The purpose of this study is to examine the robustness and sensitivity of the newly available Y-STR multiplex kit, the PowerPlex® Y23 System, by comparing our data at the 23-loci level to the routinely used 17 loci provided by the AmpFlSTR® Yfiler® PCR Amplification kit. For the first time, allelic and genotypic frequencies for the 23 Y-STR loci included in the PowerPlex® Y23 System are provided for the Southeast Florida Hispanic (SFH) population. In addition, we have characterized the SFH population in terms of intra-population and inter-population parameters. We also compared these indices of forensic and population genetics interest in the SFH population to comparable data of previously published populations to assess their phylogenetic relationships. Our 23-loci data was shown to provide more discriminatory values as compared to the data when using only 17 loci. Also, the RST distance values demonstrate the superior capacity of the PowerPlex® Y23 system to discriminate among populations.
本研究的目的是通过将我们在23个基因座水平上的数据与AmpFlSTR® Yfiler® PCR扩增试剂盒提供的常规使用的17个基因座的数据进行比较，来检验新推出的Y-STR复合试剂盒PowerPlex® Y23系统的稳健性和敏感性。首次提供了PowerPlex® Y23系统中包含的23个Y-STR基因座在佛罗里达州东南部西班牙裔（SFH）人群中的等位基因和基因型频率。此外，我们根据群体内和群体间参数对SFH人群进行了特征描述。我们还将SFH人群中这些法医和群体遗传学感兴趣的指标与先前发表的人群的可比数据进行了比较，以评估它们的系统发育关系。与仅使用17个基因座时的数据相比，我们的23个基因座数据显示出提供了更多的鉴别值。此外，RST距离值证明了PowerPlex® Y23系统在区分不同人群方面的卓越能力。
