Hu Yichun, Zhuo Qin, Gong Zhaolong, Piao Jianhua, Yang Xiaoguang
Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China.
Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China.
Food Chem Toxicol. 2017 Jan;99:190-198. doi: 10.1016/j.fct.2016.11.025. Epub 2016 Nov 24.
In the present work, we evaluated the three generation reproductive toxicity of the genetically modified rice with insectresistant cry1Ac and sck genes. 120 Sprague-Dawley (SD) rats were divided into three groups which were fed with genetically modified rice diet (GM group), parental control rice diet (PR group) and AIN-93 control diet (both used as negative control) respectively. Bodyweight, food consumption, reproductive data, hematological parameters, serum chemistry, relative organ weights and histopathology for each generation were examined respectively. All the hematology and serum chemistry parameters, organ/body weight indicators were within the normal range or no change to the adverse direction was observed, although several differences in hematology and serum chemistry parameters (WBC, BUN, LDH of male rat, PLT, PCT, MPV of female rats), reproductive data (rate of morphologically abnormal sperm) were observed between GM rice group and two control groups. No macroscopic or histological adverse effects were found or considered as treatment-related, either. Overall, the three generation study of genetically modified rice with cry1Ac and sck genes at a high level showed no unintended adverse effects on rats's reproductive system.
在本研究中，我们评估了转抗虫cry1Ac和sck基因水稻的三代生殖毒性。将120只Sprague-Dawley（SD）大鼠分为三组，分别用转基因水稻日粮（GM组）、亲本对照水稻日粮（PR组）和AIN-93对照日粮（均用作阴性对照）喂养。分别检测每一代的体重、食物摄入量、生殖数据、血液学参数、血清生化指标、相对器官重量和组织病理学。尽管在转基因水稻组和两个对照组之间观察到血液学和血清生化参数（雄性大鼠的白细胞、尿素氮、乳酸脱氢酶，雌性大鼠的血小板、血小板压积、平均血小板体积）、生殖数据（形态异常精子率）存在一些差异，但所有血液学和血清生化参数、器官/体重指标均在正常范围内，或未观察到向不利方向的变化。也未发现宏观或组织学上的不良影响，或认为与处理有关。总体而言，高水平的转cry1Ac和sck基因水稻的三代研究未显示对大鼠生殖系统有意外的不良影响。
