Bhanjadeo Madhabi M, Nayak Ashok K, Subudhi Umakanta
DNA Nanotechnology & Application Laboratory, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi 110025, India.
DNA Nanotechnology & Application Laboratory, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, India.
Biochem Biophys Res Commun. 2017 Jan 22;482(4):916-921. doi: 10.1016/j.bbrc.2016.11.133. Epub 2016 Nov 25.
DNA adopts different conformation not only because of novel base pairs but also while interacting with inorganic or organic compounds. Self-assembled branched DNA (bDNA) structures or DNA origami that change conformation in response to environmental cues hold great promises in sensing and actuation at the nanoscale. Recently, the B-Z transition in DNA is being explored to design various nanomechanical devices. In this communication we have demonstrated that Cerium chloride binds to the phosphate backbone of self-assembled bDNA structure and induce B-to-Z transition at physiological concentration. The mechanism of controlled conversion from right-handed to left-handed has been assayed by various dye binding studies using CD and fluorescence spectroscopy. Three different bDNA structures have been identified to display B-Z transition. This approach provides a rapid and reversible means to change bDNA conformation, which can be used for dynamic and progressive control at the nanoscale.
DNA不仅由于新型碱基对，而且在与无机或有机化合物相互作用时会采用不同的构象。响应环境线索而改变构象的自组装分支DNA（bDNA）结构或DNA折纸在纳米级传感和驱动方面具有巨大潜力。最近，人们正在探索DNA中的B-Z转变以设计各种纳米机械设备。在本通讯中，我们证明了氯化铈在生理浓度下与自组装bDNA结构的磷酸骨架结合并诱导B到Z的转变。通过使用圆二色光谱（CD）和荧光光谱的各种染料结合研究，分析了从右手到左手的可控转变机制。已鉴定出三种不同的bDNA结构可显示B-Z转变。这种方法提供了一种快速且可逆的手段来改变bDNA构象，可用于纳米级的动态和渐进控制。
