Teichert Ines
Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universitaet Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany.
Fungal Genet Biol. 2017 Jan;98:20-22. doi: 10.1016/j.fgb.2016.11.004. Epub 2016 Nov 23.
The ascomycete Sordaria macrospora has a long history as a model organism for studying fungal sexual development. Starting from an ascospore, sexual fruiting bodies (perithecia) develop within seven days and discharge new ascospores. Sexual development has been studied in detail, revealing genes required for perithecium formation and ascospore germination. However, the germination process per se has not yet been examined. Here I analyze nuclear dynamics during ascospore germination using a fluorescently labeled histone. Live-cell imaging revealed that nuclei are transported into germination vesicles that form on one side of the spore. Polar growth is established from these vesicles.
子囊菌大孢粪壳菌作为研究真菌有性发育的模式生物已有很长历史。从一个子囊孢子开始，有性子实体（子囊壳）在七天内发育并释放新的子囊孢子。有性发育已得到详细研究，揭示了子囊壳形成和子囊孢子萌发所需的基因。然而，萌发过程本身尚未得到研究。在这里，我使用荧光标记的组蛋白分析子囊孢子萌发过程中的核动态。活细胞成像显示，细胞核被运输到在孢子一侧形成的萌发小泡中。从这些小泡开始建立极性生长。
