Lei Xiaocan, Cui Kuiqing, Cai Xiaoyan, Ren Yanping, Liu Qingyou, Shi Deshun
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, Guangxi, China.
J Vet Med Sci. 2017 Feb 4;79(2):258-266. doi: 10.1292/jvms.16-0277. Epub 2016 Nov 25.
In the present study, we tried to determine whether bone morphogenetic protein 1 (BMP1) plays a role in ovarian follicular development and early embryo development. We systematically investigated the expression and influence of BMP1 during porcine follicle and early embryonic development. Immunohistochemistry demonstrated that the BMP1 protein is expressed in granular cells and oocytes during follicular development, from primary to pre-ovulatory follicles, including atretic follicles and the corpus luteum. The mRNA expression of BMP1 significantly increased as the porcine follicles grew. Immunofluorescence analysis indicated that BMP1 was expressed in cumulus-oocyte complexes (COCs), oocytes and porcine embryos during early in vitro culture. qPCR and western blot analysis showed that the expression of BMP1 was significantly up-regulated in mature porcine oocytes and COCs compared to immature oocytes and COCs. BMP1 is expressed in early porcine embryos, and its expression reaches a peak at the 8-cell stage. To determine the effect of BMP1 on the maturation of oocytes and the development of early embryos, various concentrations of BMP1 recombinant protein or antibody were added to the in vitro culture media, respectively. BMP1 significantly affected the porcine oocyte maturation rate, the cleavage rate and the blastocyst development rate of embryos cultured in vitro in a positive way, as well as the blastocyst cell number. In conclusion, BMP1 is expressed throughout porcine ovarian follicle development and early embryogenesis, and it promotes oocyte maturation and the developmental ability of embryos during early in vitro culture.
在本研究中，我们试图确定骨形态发生蛋白1（BMP1）是否在卵巢卵泡发育和早期胚胎发育中发挥作用。我们系统地研究了BMP1在猪卵泡和早期胚胎发育过程中的表达及影响。免疫组织化学表明，从初级卵泡到排卵前卵泡，包括闭锁卵泡和黄体，在卵泡发育过程中，颗粒细胞和卵母细胞中均有BMP1蛋白表达。随着猪卵泡的生长，BMP1的mRNA表达显著增加。免疫荧光分析表明，在早期体外培养过程中，BMP1在卵丘-卵母细胞复合体（COCs）、卵母细胞和猪胚胎中均有表达。qPCR和蛋白质免疫印迹分析表明，与未成熟卵母细胞和COCs相比，成熟猪卵母细胞和COCs中BMP1的表达显著上调。BMP1在猪早期胚胎中表达，其表达在8细胞期达到峰值。为了确定BMP1对卵母细胞成熟和早期胚胎发育的影响，分别向体外培养基中添加了不同浓度的BMP1重组蛋白或抗体。BMP1对体外培养的猪卵母细胞成熟率、胚胎的卵裂率和囊胚发育率以及囊胚细胞数量均有显著的正向影响。总之，BMP1在猪卵巢卵泡发育和早期胚胎发生过程中均有表达，并且在早期体外培养过程中促进卵母细胞成熟和胚胎的发育能力。
