狐篮子鱼（Siganus canaliculatus）（布洛赫和施奈德，1801年）成熟雌雄性腺的从头组装、特征分析及比较转录组分析

De Novo Assembly, Characterization, and Comparative Transcriptome Analysis of Mature Male and Female Gonads of Rabbitfish () (Bloch & Schneider, 1801).

作者信息

Huang Xiaolin, Huang Zhong, Li Qiang, Li Wenjun, Han Chong, Yang Yukai, Lin Heizhao, Wu Qiaer, Zhou Yanbo

机构信息

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.

National Fishery Resources and Environment Dapeng Observation and Experimental Station, Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China.

出版信息

Animals (Basel). 2024 Apr 30;14(9):1346. doi: 10.3390/ani14091346.

DOI:10.3390/ani14091346

PMID:38731350

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11083024/

Abstract

The rabbitfish, , is a commercially important table fish in southeastern China. However, there have been few studies on its gonad development and reproduction regulation. Comparative transcriptome analysis was first performed on adult male and female gonads of . In total, 47,070 unigenes were successfully assembled and 22,737 unigenes were successfully annotated. Through comparative transcriptome analysis of male and female gonads, a total of 6722 differentially expressed genes were successfully identified, with 3528 upregulated genes and 3154 downregulated genes in the testes. In addition, 39 differentially expressed reproduction-related genes were identified. Finally, quantitative real-time PCR was used to validate the expression levels of several differentially expressed genes. These results provide important data for further studying the function of reproduction-related genes and the molecular mechanism regulating gonad development and reproduction in .

摘要

篮子鱼是中国东南部一种具有重要商业价值的食用鱼。然而，关于其性腺发育和繁殖调控的研究较少。首次对篮子鱼成年雌雄性腺进行了比较转录组分析。总共成功组装了47070个单基因，成功注释了22737个单基因。通过对雌雄性腺的比较转录组分析，共成功鉴定出6722个差异表达基因，其中睾丸中有3528个上调基因和3154个下调基因。此外，还鉴定出39个差异表达的生殖相关基因。最后，采用定量实时PCR验证了几个差异表达基因的表达水平。这些结果为进一步研究篮子鱼生殖相关基因的功能以及性腺发育和繁殖的分子调控机制提供了重要数据。