Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland.
Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, Massachusetts 02115, USA, and Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, 120 University Place, Glasgow, G12 8TA, UK.
Nat Rev Microbiol. 2017 Jan;15(1):37-54. doi: 10.1038/nrmicro.2016.158. Epub 2016 Nov 28.
Over the past decade, major advances in imaging techniques have enhanced our understanding of Plasmodium spp. parasites and their interplay with mammalian hosts and mosquito vectors. Cryoelectron tomography, cryo-X-ray tomography and super-resolution microscopy have shifted paradigms of sporozoite and gametocyte structure, the process of erythrocyte invasion by merozoites, and the architecture of Maurer's clefts. Intravital time-lapse imaging has been revolutionary for our understanding of pre-erythrocytic stages of rodent Plasmodium parasites. Furthermore, high-speed imaging has revealed the link between sporozoite structure and motility, and improvements in time-lapse microscopy have enabled imaging of the entire Plasmodium falciparum erythrocytic cycle and the complete Plasmodium berghei pre-erythrocytic stages for the first time. In this Review, we discuss the contribution of key imaging tools to these and other discoveries in the malaria field over the past 10 years.
在过去的十年中，成像技术的重大进展增强了我们对疟原虫属寄生虫及其与哺乳动物宿主和疟蚊媒介相互作用的理解。冷冻电子断层扫描、冷冻 X 射线断层扫描和超分辨率显微镜改变了子孢子和配子体结构、裂殖子侵入红细胞的过程以及 Maurer 裂隙的结构的范式。活体延时成像对于我们理解啮齿动物疟原虫的红细胞前期阶段具有革命性意义。此外，高速成像揭示了子孢子结构和运动之间的联系，延时显微镜的改进使得首次能够对整个恶性疟原虫红细胞周期和整个伯氏疟原虫红细胞前期阶段进行成像。在这篇综述中，我们讨论了关键成像工具对过去 10 年中疟疾领域的这些发现和其他发现的贡献。
