Righi M, Mori L, De Libero G, Sironi M, Biondi A, Mantovani A, Donini S D, Ricciardi-Castagnoli P
CNR Center of Cytopharmacology, Milano, Italy.
Eur J Immunol. 1989 Aug;19(8):1443-8. doi: 10.1002/eji.1830190815.
Cytokines have been suggested to act as intermediates between the immune and the central nervous system, but little is known about the type of cells synthesizing them in the brain. We have immortalized with oncogenic retroviruses primary brain cell cultures from mouse embryos and have generated clones of microglial cells that have been characterized. Three of the clones studied produce interleukin 1 (IL 1); IL 6 and tumor necrosis factor-alpha as assessed by biological assays and by Northern blot analysis. Our data raise the question on the role of these cytokines in the brain and suggest that early resident microglial cells might play an important role in development processes and in the adult brain.
细胞因子被认为是免疫和中枢神经系统之间的介质，但对于大脑中合成它们的细胞类型却知之甚少。我们用致癌逆转录病毒使来自小鼠胚胎的原代脑细胞培养物永生化，并生成了已被鉴定的小胶质细胞克隆。通过生物学检测和Northern印迹分析评估，所研究的三个克隆产生白细胞介素1（IL 1）、IL 6和肿瘤坏死因子-α。我们的数据提出了这些细胞因子在大脑中的作用问题，并表明早期驻留的小胶质细胞可能在发育过程和成年大脑中发挥重要作用。
