Wong A K, Bunce C M, Lord J M, Salt J, Brown G
Department of Immunology, Medical School, University of Birmingham, Edgbaston, England.
Exp Hematol. 1989 Oct;17(9):968-73.
Various data in the literature suggest that progenitor cells of monocytes and B cells are closely related. In this study we have investigated this notion by using two-dimensional gel electrophoresis patterns of total cellular phosphoproteins to assess the similarity and thus the close relationships between erythroid, granulocyte, monocyte, and B-lymphocyte cell lines that typify immature cells of these lineages. In previous studies, six proteins had been identified whose constitutive phosphorylation correlated with the capacity of HL60 variant cell lines to differentiate towards either neutrophils (four proteins) or monocytes (two proteins). The presence or absence of five of these phosphoproteins in autoradiographs obtained for the pre-B-cell lines Nalm6 and SMSB showed that the pre-B-cell lines most closely resembled lines able to differentiate towards monocytes (HL6015-12, HL60M2, U937, and ML-1) as opposed to lines restricted to neutrophil (HL60Ast3) or erythroid differentiation (K562). The K562 phosphoprotein pattern resembled that obtained for HL60Ast3. Progressive changes in the constitutive phosphorylation of the five proteins were observed suggesting that cells that have acquired the potential for either neutrophil, monocyte, or B-cell differentiation have initially diversified in a linear progressive manner. This observation supports a model for hemopoiesis that suggests that, during progenitor cell development, differentiation potentials are expressed individually in the above order. Two additional phosphoprotein spots were found to be restricted to the pre-B-cell lines. These phosphoproteins, together with those that change their intensity in autoradiographs of erythroid, myeloid, and B-cell lines, suggest that protein phosphorylation plays an important role during cell diversification.
文献中的各种数据表明，单核细胞和B细胞的祖细胞密切相关。在本研究中，我们通过使用全细胞磷蛋白的二维凝胶电泳图谱来评估红系、粒细胞、单核细胞和B淋巴细胞系之间的相似性，从而研究这些典型的未成熟细胞系之间的密切关系。在先前的研究中，已鉴定出六种蛋白质，其组成性磷酸化与HL60变异细胞系向中性粒细胞（四种蛋白质）或单核细胞（两种蛋白质）分化的能力相关。在前B细胞系Nalm6和SMSB的放射自显影片中，这些磷蛋白中有五种的存在与否表明，前B细胞系与能够向单核细胞分化的细胞系（HL6015-12、HL60M2、U937和ML-1）最为相似，而不是局限于向中性粒细胞（HL60Ast3）或红系分化的细胞系（K562）。K562磷蛋白模式与HL60Ast3的相似。观察到这五种蛋白质的组成性磷酸化有渐进性变化，这表明已获得向中性粒细胞、单核细胞或B细胞分化潜能的细胞最初是以线性渐进方式多样化的。这一观察结果支持了一种造血模型，该模型表明，在祖细胞发育过程中，分化潜能按上述顺序依次表达。另外发现两个磷蛋白斑点仅限于前B细胞系。这些磷蛋白，连同那些在红系、髓系和B细胞系的放射自显影片中强度发生变化的磷蛋白，表明蛋白质磷酸化在细胞多样化过程中起重要作用。
