Bunce C M, Lord J M, Wong A K, Brown G
Department of Immunology, University of Birmingham, Edgbaston, UK.
Br J Cancer. 1988 Jun;57(6):559-63. doi: 10.1038/bjc.1988.128.
The human promyeloid cell line H60 can be induced to differentiate towards either neutrophils or monocytes. Variant cell lines, derived from HL60, which show reduced capacities for neutrophil and monocyte differentiation can be arranged in a developmental sequence which suggests that the potentials for neutrophil and monocyte differentiation are expressed sequentially by HL60 cells in this order. Analysis of the patterns of total cellular phosphoproteins within HL60 and 5 variant cell lines, by two-dimensional gel electrophoresis, has identified 6 distinct phosphoproteins which show progressive differences in the intensity of spots between the variant lines. The changes in these phosphoproteins relate to the position of the lines within the proposed development sequence. Similarly, lines placed close together in the sequence are more similar, as regards phosphoprotein profiles, than lines placed far apart. These studies provide direct evidence in favour of the hypothesis that the potentials for neutrophil and monocyte differentiation are expressed sequentially during myelopoiesis. Furthermore, two phosphoprotein spots were found to be restricted to lines able to differentiate towards monocytes. These proteins may play important roles during commitment to monocyte differentiation.
人早幼粒细胞系H60可被诱导分化为中性粒细胞或单核细胞。从HL60衍生而来的变异细胞系，其向中性粒细胞和单核细胞分化的能力降低，可按发育顺序排列，这表明HL60细胞按此顺序依次表达中性粒细胞和单核细胞分化的潜能。通过二维凝胶电泳分析HL60和5个变异细胞系内总细胞磷蛋白的模式，已鉴定出6种不同的磷蛋白，它们在变异系之间的斑点强度上显示出渐进差异。这些磷蛋白的变化与这些系在拟议的发育序列中的位置有关。同样，在序列中位置相近的系，就磷蛋白谱而言，比位置相距较远的系更相似。这些研究为中性粒细胞和单核细胞分化潜能在骨髓生成过程中依次表达这一假说提供了直接证据。此外，发现两个磷蛋白斑点仅限于能够向单核细胞分化的系。这些蛋白质可能在单核细胞分化的定向过程中发挥重要作用。
