使用抗甘草酸和抗甘草苷单克隆抗体，通过组合酶联免疫吸附测定法同时测定甘草根和汉方制剂中的甘草酸和甘草苷。

Simultaneous determination of glycyrrhizin and liquiritin in licorice roots and Kampo medicines by combination enzyme-linked immunosorbent assay using anti-glycyrrhizin and anti-liquiritin monoclonal antibodies.

作者信息

Fujii Shunsuke, Morinaga Osamu, Uto Takuhiro, Nomura Shuichi, Shoyama Yukihiro

机构信息

a Department of Health and Nutrition, Faculty of Health Management , Nagasaki International University , Sasebo , Japan.

b Department of Natural Medicines , Daiichi University of Pharmacy , Minami-ku , Fukuoka , Japan.

出版信息

J Immunoassay Immunochem. 2017;38(3):285-298. doi: 10.1080/15321819.2016.1260586. Epub 2016 Nov 28.

DOI:10.1080/15321819.2016.1260586

PMID:27892811

Abstract

Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines.

摘要

使用单克隆抗体（mAb）的免疫分析系统是分析、生化和临床领域中最有用的技术之一。在本研究中，开发了一种同时使用抗甘草酸和抗甘草苷单克隆抗体（抗GL/Liq混合单克隆抗体）的组合酶联免疫吸附测定（ELISA）方法，用于甘草及其制品的质量控制。该组合ELISA通过一次测定对GL和Liq的总含量显示出高灵敏度、重现性和特异性。所开发的ELISA作为从甘草属物种中筛选高质量甘草以及确认含甘草汉方药物质量的首次筛选方法是有效且有用的。