免疫层析法快速检测甘草酸
Rapid detection of glycyrrhizin by immunochromatographic assay.
作者信息
Putalun Waraporn, Tanaka Hiroyuki, Shoyama Yukihiro
机构信息
Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand.
出版信息
Phytochem Anal. 2005 Sep-Oct;16(5):370-4. doi: 10.1002/pca.862.
An immunochromatographic assay was developed for detecting glycyrrhizin (1). The qualitative assay is based on a competitive immunoassay using anti-1 monoclonal antibody (MAb) and a detector reagent that contains colloidal gold particles coated with anti-1 MAb. The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples.
开发了一种用于检测甘草甜素的免疫层析测定法(1)。该定性测定基于竞争性免疫测定，使用抗-1单克隆抗体(MAb)和一种检测试剂，该试剂含有包被有抗-1 MAb的胶体金颗粒。免疫层析条带试验的检测限为250 ng/mL，可作为检测植物、生物体液和食品样品中甘草甜素的快速筛选方法。
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