Sgorlon Sandy, Stefanon Bruno, Sandri Misa, Colitti Monica
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze, 208, 33100 Udine, Italy.
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze, 208, 33100 Udine, Italy.
Res Vet Sci. 2016 Dec;109:142-148. doi: 10.1016/j.rvsc.2016.10.005. Epub 2016 Oct 13.
The study was conducted to investigate the effects of dietary administrations of four nutraceuticals in dogs. Seventy four dogs were enrolled in the trials, 24 healthy dogs were fed with a control diet (CT) and the experimental groups received for 60days the same diet supplemented with nutraceuticals, namely Echinacea angustifolia (EA, 0.10mg/kg live weight as echinacoside; 14 dogs), Vaccinium myrtillus (VM, 0.20mg/kg live weight as anthocyanidin, 13 dogs), Curcuma longa (CL, 6.60mg/kg live weight as curcumin, 18 dogs with arthrosis), and Sylibum marianum (SM, 1.5mg/kg live weight as sylibin, 8 dogs with hepatopathy). Dogs were weighted at the beginning of study and blood samples were collected at the beginning (T0) and at the end (T60) of the study. VM significantly down regulated TNF, CXCL8, NFKB1 and PTGS2 and decreased plasma ceruloplasmin (CuCp). The activity of EA was evidenced by the significant decrease of TNF and NFKB1 expression and CuCp levels and by the increase of plasma Zn. Administration of CL caused a significant decrease of CuCp and increase of Zn and a down regulation of TNF, CXCL8, NFKB1 and PTGS2, corroborating the anti-inflammatory action of curcuminoids. After 60days of treatment with SM, plasma ALT/GPT activity was reduced and paraoxonase was increased, supporting the antioxidant activity of silymarin, also confirmed by the significant up regulation of SOD2. Results indicated that nutraceutical administrations in dogs can be an interesting approach to modulate immune response in order to improve health condition of animals.
本研究旨在调查四种营养保健品对犬类的影响。74只犬参与了试验，24只健康犬喂食对照饮食（CT），实验组在60天内喂食添加了营养保健品的相同饮食，即紫锥菊（EA，以松果菊苷计0.10mg/kg体重；14只犬）、欧洲越橘（VM，以花青素计0.20mg/kg体重，13只犬）、姜黄（CL，以姜黄素计6.60mg/kg体重，18只患有关节炎的犬）和水飞蓟宾（SM，以水飞蓟素计1.5mg/kg体重，8只患有肝病的犬）。在研究开始时对犬进行称重，并在研究开始时（T0）和结束时（T60）采集血样。VM显著下调TNF、CXCL8、NFKB1和PTGS2，并降低血浆铜蓝蛋白（CuCp）。EA的活性表现为TNF和NFKB1表达及CuCp水平显著降低，血浆锌增加。给予CL导致CuCp显著降低，锌增加，TNF、CXCL8、NFKB1和PTGS2下调，证实了姜黄素类化合物的抗炎作用。用SM治疗60天后，血浆ALT/GPT活性降低，对氧磷酶增加，支持了水飞蓟素的抗氧化活性，SOD2的显著上调也证实了这一点。结果表明，对犬类给予营养保健品可能是一种调节免疫反应以改善动物健康状况的有趣方法。
