de Godoy Maria R C, Conway Charlotte E, Mcleod Kyle R, Harmon David L
a Department of Animal and Food Sciences , University of Kentucky , Lexington , KY , USA.
Arch Anim Nutr. 2015;69(6):499-514. doi: 10.1080/1745039X.2015.1100866.
The aim of this study was to determine the effect of feeding a fish oil (FO)-containing diet on lipid and protein metabolism, postprandial glycaemia and body weight in young, lean, adult dogs. Eight female Beagles were randomly assigned to one of two isonitrogenous and isoenergetic diets, Control or FO, in a crossover design. At the beginning of the experiment and at 30 and 60 d, a baseline blood sample was collected and the dogs then were fed their daily ration. Nitrogen balance began at 07:00 h on day 63 of each experimental period and ended at 07:00 h on day 69. On day 66 of each period, a single dose (7.5 mg/kg) of (15)N-glycine was administered orally to each dog via gelatin capsule. Postprandial glycaemia did not differ between treatments or among sampling days within treatment. Cholesterol concentration was increased (p<0.05) on the Control treatment throughout the experiment when compared to values of day 0. Dogs fed the FO treatment had higher plasma triglyceride and ghrelin concentrations than those fed the Control treatment. Body weight and food intake did not differ between dietary treatments. Faecal excretion was increased (p<0.05) in the FO treatment. Dry matter digestibility was decreased (p<0.05) and fat digestibility tended (p<0.10) to decrease in the FO treatment. Overall, feeding a FO-containing diet showed a protective effect against the rise of plasma cholesterol and it increased plasma ghrelin concentration. However, FO supplementation did not appear to affect protein metabolism or postprandial glycaemia in adult lean dogs.
本研究的目的是确定在年轻、瘦体型的成年犬中，喂食含鱼油（FO）日粮对脂质和蛋白质代谢、餐后血糖及体重的影响。采用交叉设计，将8只雌性比格犬随机分为两种等氮等能量日粮组之一，即对照组或FO组。在实验开始时以及第30天和第60天，采集基线血样，然后给犬喂食其每日定量日粮。在每个实验期的第63天07:00开始氮平衡测定，并于第69天07:00结束。在每个实验期的第66天，通过明胶胶囊给每只犬口服单剂量（7.5 mg/kg）的（15）N-甘氨酸。各处理组之间以及处理组内各采样日的餐后血糖均无差异。与第0天的值相比，整个实验期间对照组的胆固醇浓度升高（p<0.05）。喂食FO处理的犬血浆甘油三酯和胃饥饿素浓度高于喂食对照组的犬。日粮处理之间的体重和采食量无差异。FO处理组的粪便排泄量增加（p<0.05）。FO处理组的干物质消化率降低（p<0.05），脂肪消化率有降低趋势（p<0.10）。总体而言，喂食含FO日粮对血浆胆固醇升高具有保护作用，并增加了血浆胃饥饿素浓度。然而，补充FO似乎并未影响成年瘦犬的蛋白质代谢或餐后血糖。
