Huang Chien-Chia, Wang Chun-Hua, Fu Chia-Hsiang, Huang Chi-Che, Chang Po-Hung, Chen Yi-Wei, Wu Chia-Chen, Wu Pei-Wen, Lee Ta-Jen
Division of Rhinology, Department of Otolaryngology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University Department of Thoracic Medicine, Chang Gung Memorial Hospital and Chang Gung Memorial Hospital and Chang Gung University, Taoyuan Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital and Chang Gung University, Keelung, Taiwan.
Medicine (Baltimore). 2016 Nov;95(47):e5432. doi: 10.1097/MD.0000000000005432.
Cigarette smoke plays a substantial role in the development of airway inflammatory diseases, including asthma and chronic rhinosinusitis (CRS). Interleukin (IL)-17A might contribute to cigarette smoke-related inflammation of the airway. This study aimed to investigate the association between cigarette smoking and IL-17A expression in the nasal tissues of patients with CRS and asthma.We prospectively recruited 24 patients (13 smokers, 11 nonsmokers) with CRS and asthma and 6 patients with asthma but without CRS (control group) in a tertiary medical center. Nasal mucosa was obtained as part of the nasal surgery. Protein and mRNA levels of IL-17A in the nasal tissues were determined by immunostaining and real-time polymerase chain reaction.The number of unexpected emergency clinic visits for acute asthma attacks were higher among smokers than among nonsmokers. Interleukin-17A protein and mRNA levels in the nasal tissues of smokers were greater compared to those in the nasal tissues of nonsmokers (P = 0.02 both) and control patients (P = 0.05 and 0.04, respectively).Cigarette smoking was associated with an increase in the number of unexpected emergency clinic visits due to acute asthma attack and in the expression of IL-17A in the nasal tissues of patients with airway inflammatory diseases.
香烟烟雾在包括哮喘和慢性鼻-鼻窦炎（CRS）在内的气道炎症性疾病的发展中起着重要作用。白细胞介素（IL）-17A可能与香烟烟雾相关的气道炎症有关。本研究旨在调查吸烟与CRS和哮喘患者鼻组织中IL-17A表达之间的关联。我们在一家三级医疗中心前瞻性招募了24例CRS合并哮喘患者（13例吸烟者，11例非吸烟者）和6例哮喘但无CRS患者（对照组）。鼻黏膜作为鼻手术的一部分获取。通过免疫染色和实时聚合酶链反应测定鼻组织中IL-17A的蛋白质和mRNA水平。吸烟者因急性哮喘发作意外急诊就诊的次数高于非吸烟者。吸烟者鼻组织中白细胞介素-17A的蛋白质和mRNA水平高于非吸烟者（两者P均=0.02）和对照组患者（分别为P=0.05和0.04）。吸烟与气道炎症性疾病患者因急性哮喘发作意外急诊就诊次数增加以及鼻组织中IL-17A表达增加有关。
