Rahman Shams, Rollison Dana E, Pierce Campbell Christine M, Waterboer Tim, Michel Angelika, Pawlita Michael, Villa Luisa L, Lazcano Ponce Eduardo, Wang Wei, Borenstein Amy R, Giuliano Anna R
Center for Infection Research in Cancer, Moffitt Cancer Center, Tampa, United States.
Department of Epidemiology and Biostatistics, College of Public Health, University of South Florida, Tampa, United States.
PLoS One. 2016 Nov 28;11(11):e0167174. doi: 10.1371/journal.pone.0167174. eCollection 2016.
A variety of cutaneous human papillomaviruses (HPV) are detectable in genital epithelial lesions in men and non-melanoma skin cancer patients. It remains unclear whether these viruses are associated causally with skin lesions. To date, no study has prospectively examined the association between cutaneous HPV seropositivity and development of external genital lesions (EGLs) in men.
To examine the association between seropositivity to cutaneous HPV types and the risk of subsequent development of EGLs.
A nested case-control study including 163 incident EGL cases and 352 EGL-free controls in the HPV Infection in Men (HIM) Study cohort was conducted. Cases were ascertained at each of up to 10 biannual clinical visits and verified through biopsy and pathological diagnoses. EGLs were categorized as condyloma, suggestive of condyloma, penile intraepithelial neoplasia (PeIN), and other EGLs. Archived serum specimens collected at baseline were tested for antibodies against 14 cutaneous HPV types (β types (5, 8, 12, 14, 17, 22, 23, 24, 38, and 47), α type 27, γ type 4, μ type 1, and ν type 41) using a GST L1-based multiplex serology assay. Socio-demographic and sexual behavior data were collected through a questionnaire. Using logistic regression, adjusted odds ratios (AOR) and 95% confidence intervals (CI) were estimated.
Overall, seropositivity to ≥1 cutaneous HPV type (any-HPV) and ≥1 β types (any-β) was 58.3% and 37.5% among other EGL cases, 71.6% and 46.8% among condyloma, 66.8% and 50.0% among PeIN, and 71.9% and 38.4% among controls, respectively. Type-specific seropositivity was most common for ɤ-HPV 4, μ-HPV 1, and β-HPV 8. No statistically significant association was observed between any-HPV, any-β, and type-specific HPV seropositivity and subsequent development of EGLs across all pathological diagnoses.
Overall, seropositivity to cutaneous HPV was common among men; however, it appears that cutaneous HPV is not associated with the development of genital lesions in men.
在男性生殖器上皮病变以及非黑色素瘤皮肤癌患者中可检测到多种皮肤型人乳头瘤病毒（HPV）。这些病毒是否与皮肤病变存在因果关系仍不清楚。迄今为止，尚无研究前瞻性地探讨男性皮肤HPV血清学阳性与外生殖器病变（EGL）发生之间的关联。
研究皮肤HPV各型血清学阳性与后续发生EGL风险之间的关联。
在男性HPV感染（HIM）研究队列中开展了一项巢式病例对照研究，纳入163例初发EGL病例和352例无EGL的对照。病例在多达10次半年一次的临床访视中确诊，并通过活检和病理诊断进行核实。EGL分为尖锐湿疣、疑似尖锐湿疣、阴茎上皮内瘤变（PeIN）和其他EGL。使用基于谷胱甘肽S-转移酶（GST）L1的多重血清学检测方法，对基线时采集的存档血清标本检测针对14种皮肤HPV型别（β型（5、8、12、14、17、22、23、24、38和47）、α27型、γ4型、μ1型和ν41型）的抗体。通过问卷调查收集社会人口统计学和性行为数据。采用逻辑回归估计调整后的比值比（AOR）和95%置信区间（CI）。
总体而言，在其他EGL病例中，≥1种皮肤HPV型别（任何HPV）血清学阳性率和≥1种β型（任何β）血清学阳性率分别为58.3%和37.5%；在尖锐湿疣中分别为71.6%和46.8%；在PeIN中分别为66.8%和50.0%；在对照中分别为71.9%和38.4%。型特异性血清学阳性在γ-HPV 4、μ-HPV 1和β-HPV 8中最为常见。在所有病理诊断中，未观察到任何HPV、任何β以及型特异性HPV血清学阳性与后续EGL发生之间存在统计学显著关联。
总体而言，男性中皮肤HPV血清学阳性很常见；然而，皮肤HPV似乎与男性生殖器病变的发生无关。
