Laboratory of Microbiology, Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
Bioresour Technol. 2017 Feb;225:225-233. doi: 10.1016/j.biortech.2016.11.024. Epub 2016 Nov 19.
Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and β-glucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44% of pectin derived sugars.
精疲力竭的糖甜菜短节（ESBC）作为生产生物制品的糖源具有巨大的潜力。用主要的纤维素酶、木聚糖酶和果胶酶联合作用的酶水解，结果非常有效，与单独使用 Celluclast®和β-葡萄糖苷酶测量的糖浓度几乎提高了一倍，并将水解速率提高了 5 倍。作为唯一的预处理方法，ESBC 在水解缓冲液中浸泡并高压灭菌，避免了应用严格的常规生物质预处理方法。此外，本文提出了一种很有前途的方法，可以充分利用 ESBC 中所含的葡萄糖、木糖、阿拉伯糖、甘露糖和麦芽糖。它包括在水解步骤中释放的糖的顺序发酵，以生产生物乙醇和乳酸作为主要的生物产品。与单独发酵相比，采用这种策略，葡萄糖和半纤维素衍生糖被完全消耗，果胶衍生糖的 44%被消耗。
