Department of Chemical Engineering and Food Technology, Faculty of Sciences, IVAGRO, University of Cádiz, Spain.
Waste Manag Res. 2019 Feb;37(2):149-156. doi: 10.1177/0734242X18798699. Epub 2018 Sep 17.
Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg and 17,600 U·kg, respectively; while for ESBC the maximum values reached were 35,000 U·kg and 28,000 U·kg, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.
如今，大量的农业工业废物被工业企业丢弃；然而，它们却是生产高附加值产品的有趣原料。在这方面，橙皮（ORA）和废糖蜜棉籽（ESBC）已被证明是通过固态发酵（SSF）生产水解酶的有前途的原料，也是可以发酵成不同高附加值产品的糖的来源。在 SSF 橙皮后获得的酶提取物中，木聚糖酶和外聚半乳糖醛酸酶（exo-PG）的最大活性分别为 31000 U·kg 和 17600 U·kg；而 ESBC 的最大活性分别为 35000 U·kg 和 28000 U·kg。在 SSF 实验中获得的酶提取物也被用于 ORA 和 ESBC 的水解。此外，还发现 SSF 橙皮提取物补充商业纤维素酶后，比通常用于此目的的商业酶混合物更有效地水解 ORA 和 ESBC。在这种情况下，ESBC 和 ORA 的酶解后，最大还原糖浓度分别为 57 和 47 g·L。
