Zeng Guangping, Zhang Deying, Liu Xing, Kang Qing, Fu Yiyao, Tang Bo, Guo Wenhao, Zhang Yuanyuan, Wei Guanghui, He Dawei
Department of Urology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China.
Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27103, USA.
Oncotarget. 2017 Jan 17;8(3):4607-4617. doi: 10.18632/oncotarget.13491.
This study aimed to explore the localization and expression of P-element-induced wimpy testis-like 2 (piwil2)/Piwil4 in hepatocellular carcinoma (HCC) tissues, and analyze the correlation between co-expression pattern and prognosis of HCC.
Piwil2 showed 100% positive expression in the cell nucleus, with the intensity higher than in the cytoplasm. Piwil4 showed a lower intensity of expression in the cell nucleus than in the cytoplasm. The molecular chaperone Piwil2/Piwil4 had four co-expression patterns: nuclear co-expression, nuclear and cytoplasmic co-expression, cytoplasmic co-expression, and non-coexpression. The survival rate and the overall survival sequentially increased. The prognostic phenotype of the nuclear co-expression of Piwil2/Piwil4 was worse than that of non-coexpression, and the intracellular localization and expression of Piwil2 and Piwil4 were not significantly different.
HCC pathological tissue samples with follow-up information (90 cases) and 2 normal control liver tissues were collected and made into a 92-site microarray. The expression of Piwil2 and Piwil4 was detected using the immunofluorescence double staining method. The differences in the expression and location of Piwil2 and Piwil4 in tumor cells were explored, and the influence of such differences on the long-term survival rate of HCC was studied using Kaplan-Meier survival curve and log-rank test. The clinical staging was analyzed according to the HCC international TNM staging criteria.
The nuclear co-expression of Piwil2/Piwil4 indicated that patients with HCC had a worse prognostic phenotype. The molecular chaperone Piwil2/Piwil4 seems promising as a molecular marker for prognosis judgment; a single marker (Piwil2/Piwil4) cannot be used for prognosis judgment.
本研究旨在探讨P元件诱导的类弱精睾丸2（piwil2）/Piwil4在肝细胞癌（HCC）组织中的定位与表达，并分析其共表达模式与HCC预后的相关性。
Piwil2在细胞核中呈100%阳性表达，强度高于细胞质。Piwil4在细胞核中的表达强度低于细胞质。分子伴侣Piwil2/Piwil4有四种共表达模式：核共表达、核与细胞质共表达、细胞质共表达和非共表达。生存率和总生存期依次增加。Piwil2/Piwil4核共表达的预后表型比非共表达差，且Piwil2和Piwil4的细胞内定位和表达无显著差异。
收集具有随访信息的HCC病理组织样本（90例）和2例正常对照肝组织，制成92位点微阵列。采用免疫荧光双染色法检测Piwil2和Piwil4的表达。探讨Piwil2和Piwil4在肿瘤细胞中表达和定位的差异，并采用Kaplan-Meier生存曲线和对数秩检验研究这些差异对HCC长期生存率的影响。根据HCC国际TNM分期标准分析临床分期。
Piwil2/Piwil4的核共表达表明HCC患者的预后表型较差。分子伴侣Piwil2/Piwil4作为预后判断的分子标志物似乎很有前景；单一标志物（Piwil2/Piwil4）不能用于预后判断。
