重组人肿瘤坏死因子β（淋巴毒素）对人外周血中性粒细胞、单核细胞和T细胞缺乏趋化活性。
Recombinant human tumour necrosis factor beta (lymphotoxin) lacks chemotactic activity for human peripheral blood neutrophils, monocytes, and T cells.
作者信息
Mrowietz U, Ternowitz T, Schröder J M, Christophers E
机构信息
Department of Dermatology, University of Kiel, FRG.
出版信息
Scand J Immunol. 1989 Sep;30(3):373-7. doi: 10.1111/j.1365-3083.1989.tb01224.x.
Human recombinant tumour necrosis factor beta (rhuTNF beta)/lymphotoxin was tested for human neutrophil granulocyte (PMN), monocytes (MO), and T-cell chemotactic activity by means of a modified Boyden chamber system. Over a wide range of concentrations (10(-7)-10(-14)M)rhuTNF beta showed no chemotactic activity for PMN, MO, or T cells. In contrast, strong chemotactic migration was elicited in PMN and MO with the tripeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) and in T cells when complement split product C5a and leukotriene B4 (LTB4) were used as chemotaxins. The results of this study indicate that rhuTNF beta/lymphotoxin is not a chemotaxin for human PMN, MO, or T lymphocytes in vitro.
通过改良的博伊登小室系统，对人重组肿瘤坏死因子β（rhuTNFβ）/淋巴毒素进行了人中性粒细胞（PMN）、单核细胞（MO）和T细胞趋化活性的测试。在很宽的浓度范围（10⁻⁷ - 10⁻¹⁴M）内，rhuTNFβ对PMN、MO或T细胞均未表现出趋化活性。相比之下，当使用三肽N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）时，PMN和MO会出现强烈的趋化迁移，而当使用补体裂解产物C5a和白三烯B4（LTB4）作为趋化因子时，T细胞会出现强烈的趋化迁移。本研究结果表明，rhuTNFβ/淋巴毒素在体外并非人PMN、MO或T淋巴细胞的趋化因子。
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