使用基于量子点的条形码分析法对感染性细菌进行无抗体检测。
Antibody-free detection of infectious bacteria using quantum dots-based barcode assay.
作者信息
Cihalova Kristyna, Hegerova Dagmar, Jimenez Ana Maria, Milosavljevic Vedran, Kudr Jiri, Skalickova Sylvie, Hynek David, Kopel Pavel, Vaculovicova Marketa, Adam Vojtech
机构信息
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
出版信息
J Pharm Biomed Anal. 2017 Feb 5;134:325-332. doi: 10.1016/j.jpba.2016.10.025. Epub 2016 Nov 5.
Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 10 CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).
金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和肺炎克雷伯菌是引起传染病最具代表性的细菌。由于抗生素使用的增加，细菌耐药性不断增强，导致严重并发症。因此，对这些病原体进行灵敏检测对于有效治疗至关重要。本研究的目的是利用磁性颗粒和荧光纳米颗粒（量子点）的特性，设计一种有效检测金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和肺炎克雷伯菌的多重检测方法。该方法利用细菌特异性基因（fnbA、mecA和wcaG）能够检测低至10 CFU/mL的细菌浓度。
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