Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA Deemed University, Thanjavur, 613 401, India.
School of Electrical & Electronics Engineering (SEEE), SASTRA Deemed University, Thanjavur, 613 401, India.
Sci Rep. 2019 Nov 22;9(1):17348. doi: 10.1038/s41598-019-53979-5.
According to the World Health Organization (WHO), cardiovascular disease (CVD) is the leading cause of death in the world every year. The design and development of biosensors for the detection of CVD markers could be one of the major contributions of the scientific community to society. In this context, acetic acid functionalized graphene quantum dots (fGQDs) were used as an interface for the electrochemical detection of cardiac Troponin I (cTnI). The interaction of cTnI with fGQDs for the early diagnosis of acute myocardial infarction was investigated using cyclic voltammetry (CV) and amperometry. The carbodiimide conjugation between the N-H group of cTnI and the functionalized COOH group on GQDs enabled the detection of cTnI biomarker. The same sensing mechanism was confirmed using Fourier Transform Infrared Spectrometry (FTIR). The fGQDs modified Au electrode showed remarkable electrocatalytic oxidation of cTnI with good stability and sensitivity over a linear range of 0.17 to 3 ng mL and a low detection limit of 0.02 ng mL. Bland-Altman plots substantiate a bias between the intra-/inter-cTnI assay and calibrated cTnI assay with 95% limits of agreement (mean difference ± 1.96 SD). The aim of this study is to describe an innovative method to detect cardiac biomarker cTnI and provide preliminary data on its diagnostic capacity. At the same time, its applicability in clinical setting will have to be validated with a significant number of samples collected from patients.
根据世界卫生组织（WHO）的数据，心血管疾病（CVD）每年都是世界上的主要死因。设计和开发用于检测 CVD 标志物的生物传感器可能是科学界为社会做出的主要贡献之一。在这种情况下，使用乙酸功能化石墨烯量子点（fGQDs）作为电化学检测心肌肌钙蛋白 I（cTnI）的界面。使用循环伏安法（CV）和安培法研究了 cTnI 与 fGQDs 的相互作用，以用于急性心肌梗死的早期诊断。cTnI 的 N-H 基团与 GQDs 上的功能化 COOH 基团之间的碳二亚胺偶联使 cTnI 生物标志物得以检测。傅里叶变换红外光谱（FTIR）也证实了相同的传感机制。修饰后的 Au 电极上的 fGQDs 对 cTnI 表现出显著的电催化氧化作用，具有良好的稳定性和在 0.17 到 3ngmL 的线性范围内的灵敏度，检测限低至 0.02ngmL。Bland-Altman 图证实了内/间 cTnI 测定法与经校准的 cTnI 测定法之间的偏差，95%的一致性界限（平均差异 ± 1.96 SD）。本研究的目的是描述一种用于检测心脏生物标志物 cTnI 的创新方法，并提供其诊断能力的初步数据。同时，还必须通过从患者中收集大量样本来验证其在临床环境中的适用性。
