Ajiboye T O, Hussaini A A, Nafiu B Y, Ibitoye O B
Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
J Ethnopharmacol. 2017 Feb 23;198:184-193. doi: 10.1016/j.jep.2016.11.043. Epub 2016 Nov 26.
Hunteria umbellata is used in the management and treatment of diabetes and obesity in Nigeria. This study evaluates the effect of aqueous seed extract of Hunteria umbellata on insulin resistance, dyslipidemia, inflammation and oxidative stress in high-fructose diet-induced metabolic syndrome MATERIALS AND METHODS: Rats were randomized into seven groups (A-G). Control (group A) and group C rats received control diet for nine weeks while rats in groups B, D - G were placed on high-fructose diet for 9 weeks. In addition to the diets, groups C - F rats orally received 400, 100, 200 and 400mg/kg body weight aqueous seed extract of Hunteria umbellata for 3 weeks starting from 6th - 9th week.
High-fructose diet (when compared to control rats) mediated a significant (p<0.05) increase in body weight, body mass index and abdominal circumference. Similarly, levels of blood glucose, insulin, leptin, adiponectin and insulin resistance were increased. It also caused a significant increase in the levels of cholesterol, triglycerides, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, cardiac index and coronary artery index while high-density lipoprotein cholesterol was decreased significantly. Levels of proinflammatory factor, tumour necrosis factor-α, interleukin-6 and 8 were also increased by the high fructose diet. Moreover, it mediated decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase and level of glutathione reduced. Conversely, levels of malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and fragmented DNA were elevated. Aqueous seed extract of Hunteria umbellata significantly ameliorated the high fructose diet-mediated alterations.
From this study, it is concluded that aqueous seed extract of Hunteria umbellata possesses hypoglycemic, hypolipidemic and antioxidants abilities as evident from its capability to extenuate insulin resistance, dyslipidemia, inflammation and oxidative stress in high-fructose diet-induced metabolic syndrome rats.
在尼日利亚，伞花非洲萝芙木被用于糖尿病和肥胖症的管理与治疗。本研究评估了伞花非洲萝芙木种子水提取物对高果糖饮食诱导的代谢综合征中胰岛素抵抗、血脂异常、炎症和氧化应激的影响。
将大鼠随机分为七组（A - G）。对照组（A组）和C组大鼠接受对照饮食9周，而B、D - G组大鼠接受高果糖饮食9周。除饮食外，C - F组大鼠从第6 - 9周开始口服400、100、200和400mg/kg体重的伞花非洲萝芙木种子水提取物，持续3周。
与对照大鼠相比，高果糖饮食使体重、体重指数和腹围显著增加（p<0.05）。同样，血糖、胰岛素、瘦素、脂联素水平和胰岛素抵抗也升高。它还导致胆固醇、甘油三酯、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、动脉粥样硬化指数、心脏指数和冠状动脉指数水平显著增加，而高密度脂蛋白胆固醇显著降低。高果糖饮食还使促炎因子、肿瘤坏死因子-α、白细胞介素-6和8水平升高。此外，它导致超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、葡萄糖6 - 磷酸脱氢酶活性降低以及还原型谷胱甘肽水平降低。相反，丙二醛、共轭二烯、脂质氢过氧化物、蛋白质羰基和断裂DNA水平升高。伞花非洲萝芙木种子水提取物显著改善了高果糖饮食介导的改变。
从本研究可以得出结论，伞花非洲萝芙木种子水提取物具有降血糖、降血脂和抗氧化能力，这从其减轻高果糖饮食诱导的代谢综合征大鼠的胰岛素抵抗、血脂异常、炎症和氧化应激的能力中得以体现。
