Ibitoye O B, Ghali U M, Adekunle J B, Uwazie J N, Ajiboye T O
Antioxidants, Redox Biology and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
Evid Based Complement Alternat Med. 2017;2017:8128125. doi: 10.1155/2017/8128125. Epub 2017 Oct 23.
(Stapf) Diels leaves are widely used in the treatment of diabetes, obesity, and cardiovascular related complications in Nigeria. This study investigates the anti-inflammatory and antiobesity effect of aqueous extract of leaves in high-fat diet- (HFD-) induced obese rats. HFD-fed rats were given 100, 200, and 400 mgkg body weight of aqueous extract of leaves for 4 weeks starting from 9th week of HFD treatment. leaves aqueous extract reversed HFD-mediated decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glucose 6-phosphate dehydrogenase. Moreover, HFD-mediated elevation in the levels of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl, and DNA fragmentation in rats liver was lowered. HFD-mediated alterations in serum total cholesterol, triacylglycerol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol were significantly reversed by the extract. The treatment of HFD-fed rats reduced the levels of insulin, leptin, protein carbonyl, fragmented DNA, and tumour necrosis factor- and interleukin- (IL-) 6 and IL- 8 and increased the adiponectin level. This study showed that aqueous extract of leaves has potential antiobesity and anti-inflammatory effects through modulation of obesity-induced inflammation, oxidative stress, and obesity-related disorder in HFD-induced obese rats.
（施塔普夫）在尼日利亚，迪尔斯叶被广泛用于治疗糖尿病、肥胖症以及心血管相关并发症。本研究调查了迪尔斯叶水提取物对高脂饮食（HFD）诱导的肥胖大鼠的抗炎和抗肥胖作用。从高脂饮食治疗的第9周开始，给高脂饮食喂养的大鼠连续4周给予100、200和400毫克/千克体重的迪尔斯叶水提取物。迪尔斯叶水提取物逆转了高脂饮食介导的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和葡萄糖6-磷酸脱氢酶活性的降低。此外，高脂饮食介导的大鼠肝脏中共轭二烯、脂质氢过氧化物、丙二醛、蛋白质羰基和DNA片段化水平的升高也有所降低。提取物显著逆转了高脂饮食介导的血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇的变化。对高脂饮食喂养的大鼠进行治疗降低了胰岛素、瘦素、蛋白质羰基、片段化DNA、肿瘤坏死因子-α和白细胞介素-6以及白细胞介素-8的水平，并提高了脂联素水平。本研究表明，迪尔斯叶水提取物通过调节高脂饮食诱导的肥胖大鼠中肥胖诱导的炎症、氧化应激和肥胖相关疾病，具有潜在的抗肥胖和抗炎作用。
