Zhou Bo, Wang Gongping, Gao Shegan, Chen Ye, Jin Canhui, Wang Zengfang, Yang Yantong, Ma Zhikun, Zhang Wei, Feng Xiaoshan
Department of Oncology Surgery, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Oncol Lett. 2016 Nov;12(5):3936-3940. doi: 10.3892/ol.2016.5215. Epub 2016 Sep 29.
The present study aimed to investigate the expression of Golgi phosphoprotein-3 (GOLPH3) protein in colon cancer tissues and the association with the prognosis of patients. In total, 98 patients with colon cancer admitted to The First Affiliated Hospital of Henan University of Science and Technology for surgery between June 2011 and June 2013 were taken as the observation group. In addition, 15 healthy individuals, determined by enteroscopy, were taken as the control group. The expressions of GOLPH3 mRNA and protein were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively. The patients were divided into GOLPH3-positive and GOLPH3-negative groups according to the expression of GOLPH3. The expression of GOLPH3 in colon cancer and its association with the prognosis of patients was analyzed. The expression of GOLPH3 mRNA and protein in colon cancer tissues was significantly increased compared with normal colon mucosa (P<0.05); among the tissues, GOLPH3 was not expressed in 29 patients and positively expressed in 69 patients. The expression of GOLPH3 was negatively associated with the tumor differentiation degree, and positively associated with tumor invasion depth, lymph node metastasis and clinical stages in GOLPH3-positive patients. The cumulative recurrence rates at 1, 2 and 3 years were significantly lower in GOLPH3-negative patients (P<0.05). The survival rates at 1, 2 and 3 years in the GOLPH3-positive group were significantly higher than that of the GOLPH3-negative patients (P<0.05). In conclusion, the positive expression of GOLPH3 mRNA and protein in colon cancer tissue was significantly increased compared with the control group. GOLPH3 expression was closely associated with the pathological features, consisting of tissue typing, clinical stage, degree of tumor invasion and lymph node metastasis, and GOLPH3 expression. Patients with GOLPH3 overexpression also had a poor prognosis.
本研究旨在探讨高尔基体磷蛋白3（GOLPH3）蛋白在结肠癌组织中的表达及其与患者预后的关系。选取2011年6月至2013年6月在河南科技大学第一附属医院接受手术治疗的98例结肠癌患者作为观察组。另外，选取经肠镜检查确定的15例健康个体作为对照组。分别采用逆转录-聚合酶链反应和免疫组织化学法检测GOLPH3 mRNA和蛋白的表达。根据GOLPH3的表达情况将患者分为GOLPH3阳性组和GOLPH3阴性组。分析结肠癌中GOLPH3的表达及其与患者预后的关系。与正常结肠黏膜相比，结肠癌组织中GOLPH3 mRNA和蛋白的表达显著升高（P<0.05）；在这些组织中，29例患者GOLPH3未表达，69例患者呈阳性表达。GOLPH3的表达与肿瘤分化程度呈负相关，在GOLPH3阳性患者中与肿瘤浸润深度、淋巴结转移及临床分期呈正相关。GOLPH3阴性患者1、2和3年的累积复发率显著较低（P<0.05）。GOLPH3阳性组1、2和3年的生存率显著高于GOLPH3阴性患者（P<0.05）。综上所述，与对照组相比，结肠癌组织中GOLPH3 mRNA和蛋白的阳性表达显著增加。GOLPH3的表达与包括组织分型、临床分期、肿瘤浸润程度和淋巴结转移在内的病理特征密切相关，且GOLPH3表达与患者预后相关。GOLPH3过表达的患者预后也较差。
