利用双光子吸收对含有β-肽基-7-硝基吲哚啉单元的肽进行光解。
Photolysis of a peptide with -peptidyl-7-nitroindoline units using two-photon absorption.
作者信息
Hatch Kevin A, Ornelas Alfredo, Williams Kaitlyn N, Boland Thomas, Michael Katja, Li Chunqiang
机构信息
Department of Physics, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
Department of Chemistry, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
出版信息
Biomed Opt Express. 2016 Oct 19;7(11):4654-4659. doi: 10.1364/BOE.7.004654. eCollection 2016 Nov 1.
-acyl-7-nitroindolines have been used as caged compounds to photorelease active molecules by a one- or two-photon excitation mechanism in biological systems. Here, we report the photolysis of a polypeptide that contains 7-nitroindoline units as linker moieties in its peptide backbone for potential materials engineering applications. Upon two-photon excitation with femtosecond laser light at 710 nm the photoreactive amide bond in -peptidyl-7-nitroindolines is cleaved rendering short peptide fragments. Thus, this photochemical process changes the molecular composition at the laser focal volume. Gel modifications of this peptide can potentially be used for three-dimensional microstructure fabrication.
酰基-7-硝基吲哚啉已被用作笼形化合物，通过单光子或双光子激发机制在生物系统中光释放活性分子。在此，我们报道了一种多肽的光解，该多肽在其肽主链中含有7-硝基吲哚啉单元作为连接部分，用于潜在的材料工程应用。用710nm的飞秒激光进行双光子激发时，-肽基-7-硝基吲哚啉中的光反应性酰胺键被裂解，产生短肽片段。因此，这种光化学过程改变了激光聚焦体积处的分子组成。这种肽的凝胶修饰有可能用于三维微观结构制造。
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