Zhang Huamin, Wu Junqing, Dai Zihui, Qin Meiling, Hao Lingyu, Ren Yanjing, Li Qingfei, Zhang Lugang
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Theor Appl Genet. 2017 Mar;130(3):539-547. doi: 10.1007/s00122-016-2833-9. Epub 2016 Nov 28.
In Chinese cabbage, there are two Rf loci for pol CMS and one of them was mapped to a 12.6-kb region containing a potential candidate gene encoding PPR protein. In Chinese cabbage (Brassica rapa), polima cytoplasmic male sterility (pol CMS) is an important CMS type and is widely used for hybrid breeding. By extensive test crossing in Chinese cabbage, four restorer lines (92s105, 01s325, 00s109, and 88s148) for pol CMS were screened. By analyzing the allelism of the four restorer lines, it was found that 92s105, 01s325, and 00s109 had the same "restorers of fertility" (Rf) locus (designated as BrRfp1), but 88s148 had a different Rf locus (designated as BrRfp2). For fine mapping the BrRfp1 locus of 92s105, a BCF population with 487 individuals and a BCF population with 2485 individuals were successively constructed. Using simple sequence repeat (SSR) markers developed from Brassica rapa reference genome and InDel markers derived from whole-genome resequencing data of 94c9 and 92s105, BrRfp1 was mapped to a 12.6-kb region containing a potential candidate gene encoding pentatricopeptide repeat-containing protein. Based on the nucleotide polymorphisms of the candidate gene sequence between the restoring and nonrestoring alleles, a co-segregating marker SC718 was developed, which would be helpful for hybrid breeding by marker-assisted screening and for detecting new restorer lines.
在大白菜中，存在两个与pol细胞质雄性不育（pol CMS）相关的育性恢复基因座（Rf），其中一个被定位到一个12.6千碱基对的区域，该区域包含一个潜在的候选基因，编码PPR蛋白。在大白菜（Brassica rapa）中，波里马细胞质雄性不育（pol CMS）是一种重要的细胞质雄性不育类型，广泛应用于杂交育种。通过在大白菜中进行广泛的测交，筛选出了四个pol CMS的恢复系（92s105、01s325、00s109和88s148）。通过分析这四个恢复系的等位性，发现92s105、01s325和00s109具有相同的“育性恢复基因”（Rf）位点（命名为BrRfp1），但88s148具有不同的Rf位点（命名为BrRfp2）。为了精细定位92s105的BrRfp1位点，先后构建了一个包含487个个体的回交群体（BCF）和一个包含2485个个体的回交群体。利用从甘蓝型油菜参考基因组开发的简单序列重复（SSR）标记以及从94c9和92s105的全基因组重测序数据中获得的插入缺失（InDel）标记，BrRfp1被定位到一个12.6千碱基对的区域，该区域包含一个潜在的候选基因，编码含五肽重复序列的蛋白。基于候选基因序列在恢复和非恢复等位基因之间的核苷酸多态性，开发了一个共分离标记SC718，这将有助于通过标记辅助筛选进行杂交育种以及检测新的恢复系。
