Zhang Wei, Shi Haoran, Zhou Ying, Liang Xingyu, Luo Xuan, Xiao Chaowen, Li Yun, Xu Peizhou, Wang Jisheng, Gong Wanzhuo, Zou Qiong, Tao Lanrong, Kang Zeming, Tang Rong, Li Zhuang, Yang Jin, Fu Shaohong
Chengdu Academy of Agricultural and Forestry Sciences, Chengdu, China.
Chengdu Research Branch, National Rapeseed Genetic Improvement Center, Chengdu, China.
Front Plant Sci. 2022 Apr 26;13:871006. doi: 10.3389/fpls.2022.871006. eCollection 2022.
When homozygously fertile plants were induced using doubled haploid (DH) induction lines Y3380 and Y3560, the morphology of the induced F generation was basically consistent with the female parent, but the fertility was separated, showing characteristics similar to cytoplasmic male sterile (CMS) and maintainer lines. In this study, the morphology, fertility, ploidy, and cytoplasm genotype of the induced progeny were identified, and the results showed that the sterile progeny was cytoplasm sterile ( CMS) and the fertile progeny was cytoplasm. The molecular marker and test-cross experimental results showed that the fertile progeny did not carry the restorer gene of CMS and the genetic distance between the female parent and the offspring was 0.002. This suggested that those inductions which produced sterile and fertile progeny were coordinated to CMS and maintainer lines. Through the co-linearity analysis of the mitochondrial DNA (mtDNA), it was found that the rearrangement of mtDNA by DH induction was the key factor that caused the transformation of fertility () into sterility (). Also, when heterozygous females were induced with DH induction lines, the induction F generation also showed the segregation of fertile and sterile lines, and the genetic distance between sterile and fertile lines was approximately 0.075. Therefore, the induction line can induce different types of female parents, and the breeding of the sterile line and the maintainer line can be achieved through the rapid synchronization of sister crosses and self-crosses. The induction of DH inducer in can provide a new model for the innovation of germplasm resources and open up a new way for its application.
利用双单倍体（DH）诱导系Y3380和Y3560诱导纯合可育植株时，诱导的F代形态基本与母本一致，但育性出现分离，表现出类似细胞质雄性不育（CMS）和保持系的特征。本研究对诱导后代的形态、育性、倍性和细胞质基因型进行了鉴定，结果表明不育后代为细胞质不育（CMS），可育后代为细胞质可育。分子标记和测交实验结果表明，可育后代不携带CMS的恢复基因，母本与后代之间的遗传距离为0.002。这表明那些产生不育和可育后代的诱导与CMS和保持系是协同的。通过线粒体DNA（mtDNA）的共线性分析，发现DH诱导导致的mtDNA重排是导致育性（可育）向不育转变的关键因素。此外，用DH诱导系诱导杂合母本时，诱导的F代也表现出可育系和不育系的分离，不育系和可育系之间的遗传距离约为0.075。因此，诱导系可以诱导不同类型的母本，通过姊妹交和自交的快速同步可以实现不育系和保持系的选育。DH诱导剂在该研究中的应用可为种质资源创新提供新的模式，为其应用开辟新途径。
