de Matos Ricardo Almeida, Courrol Lilia Coronato
Department of Exact and Earth Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
Amino Acids. 2017 Feb;49(2):379-388. doi: 10.1007/s00726-016-2371-4. Epub 2016 Nov 28.
The synthesis of nanoparticles is usually carried out by chemical reduction, which is effective but uses many toxic substances, making the process potentially harmful to the environment. Hence, as part of the search for environmentally friendly or green synthetic methods, this study aimed to produce silver nanoparticles (AgNPs) using only AgNO, Milli-Q water, white light from a xenon lamp (Xe) and amino acids. Nanoparticles were synthetized using 21 amino acids, and the shapes and sizes of the resultant nanoparticles were evaluated. The products were characterized by UV-Vis, zeta potential measurements and transmission electron microscopy. The synthesis of silver nanoparticles with tryptophan and tyrosine, methionine, cystine and histidine was possible through photoreduction method. Spherical nanoparticles were produced, with sizes ranging from 15 to 30 nm. Tryptophan does not require illumination nor heating, and the solution color changes immediately after the mixing of reagents if sodium hydroxide is added to the solution (pH = 10). The Xe illumination acts as sodium hydroxide in the nanoparticles synthesis, releases H and allows the reduction of silver ions (Ag) in metallic silver (Ag).
纳米颗粒的合成通常通过化学还原法进行,这种方法有效,但使用了许多有毒物质,使得该过程可能对环境有害。因此,作为寻找环境友好型或绿色合成方法的一部分,本研究旨在仅使用硝酸银、超纯水、氙灯发出的白光和氨基酸来制备银纳米颗粒(AgNPs)。使用21种氨基酸合成了纳米颗粒,并对所得纳米颗粒的形状和尺寸进行了评估。通过紫外可见光谱、zeta电位测量和透射电子显微镜对产物进行了表征。通过光还原法可以用色氨酸、酪氨酸、蛋氨酸、胱氨酸和组氨酸合成银纳米颗粒。制备出了球形纳米颗粒,尺寸范围为15至30纳米。色氨酸不需要光照和加热,如果向溶液(pH = 10)中加入氢氧化钠,试剂混合后溶液颜色会立即改变。在纳米颗粒合成中,氙灯照射起到了氢氧化钠的作用,释放出氢气并使银离子(Ag⁺)还原为金属银(Ag)。