Dornelles Guilherme Lopes, Bueno Andressa, de Oliveira Juliana Sorraila, da Silva Aleksandro Schafer, França Raqueli Teresinha, da Silva Cássia Bagolin, Machado Márcia Silveira Netto, Petry Letícia do Santos, Abdalla Fátima Husein, Lhamas Cibele Lima, de Andrade Cinthia Melazzo
Department of Small Animal Clinic, Veterinary Hospital, Universidade Federal de Santa Maria, Av. Roraima, 1000, Santa Maria, 97105-900, RS, Brazil.
Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil.
Mol Cell Biochem. 2017 Jan;425(1-2):181-189. doi: 10.1007/s11010-016-2872-1. Epub 2016 Nov 28.
The objective of this study was to evaluate the effects of different protocols (P1, P2, and P3) of boldenone undecylenate (BU) and stanozolol (ST) on markers of liver and kidney function and variables of oxidative stress in these organs. For this, 54 male Wistar rats were divided into nine groups of six animals each. Each animal received intramuscularly 5.0 mg kg of BU or ST once a week for 4 weeks (P1); 2.5 mg kg of BU or ST once a week for 8 weeks (P2); and 1.25 mg kg of BU or ST once a week for 12 weeks (P3). For each protocol, a control group was used, and they received 0.1 ml of olive oil intramuscularly. Blood and fragments of liver and kidney were collected for alanine aminotransferase activity (ALT), alkaline phosphatase, albumin, creatinine, cholesterol, total protein, triglycerides, urea, reactive oxygen species, thiobarbituric acid reactive substances, total thiols, and glutathione evaluation. The results show that the BU in doses of 5 (day 30) and 2.5 mg kg (day 60) changes the ALT seric activity, possibly showing a hepatotoxic effect. High doses of BU may lead to increased levels of cholesterol (protocol P1) possibly due to inhibition of the normal steroid biosynthesis process. All protocols used caused changes in the redox balance of the organs studied (except in the liver, protocol P2), which indicates that these drugs might be harmful even at low doses.
本研究的目的是评估不同方案（P1、P2和P3）的十一酸睾酮（BU）和司坦唑醇（ST）对肝功能和肾功能标志物以及这些器官氧化应激变量的影响。为此，将54只雄性Wistar大鼠分为9组，每组6只动物。每只动物每周肌肉注射5.0mg/kg的BU或ST，持续4周（P1）；每周肌肉注射2.5mg/kg的BU或ST，持续8周（P2）；每周肌肉注射1.25mg/kg的BU或ST，持续12周（P3）。对于每个方案，使用一个对照组，它们肌肉注射0.1ml橄榄油。采集血液以及肝脏和肾脏组织碎片，用于评估丙氨酸转氨酶活性（ALT）、碱性磷酸酶、白蛋白、肌酐、胆固醇、总蛋白、甘油三酯、尿素、活性氧、硫代巴比妥酸反应性物质、总硫醇和谷胱甘肽。结果表明，5mg/kg（第30天）和2.5mg/kg（第60天）剂量的BU会改变血清ALT活性，可能显示出肝毒性作用。高剂量的BU可能导致胆固醇水平升高（方案P1），这可能是由于抑制了正常的类固醇生物合成过程。所有使用的方案都会导致所研究器官的氧化还原平衡发生变化（肝脏的方案P2除外），这表明即使是低剂量这些药物也可能有害。
