给去卵巢并用果糖喂养的斯普拉格-道利大鼠摄入富含天然矿物质的水：对沉默调节蛋白1和糖皮质激素信号通路的影响。

Natural mineral-rich water ingestion by ovariectomized fructose-fed Sprague-Dawley rats: effects on sirtuin 1 and glucocorticoid signaling pathways.

作者信息

Das Jugal Kishore, Severo Milton, Pereira Cidália Dionísio, Patrício Emília, Magalhães José, Monteiro Rosário, Neves Delminda, Martins Maria João

机构信息

1Department of Biochemistry, Faculty of Medicine, University of Porto, Porto, Portugal 2School of Biotechnology, KIIT University, Bhubaneswar, India 3Department of Clinical Epidemiology, Predictive Medicine and Public Health, Faculty of Medicine, University of Porto, Porto, Portugal 4Unidade de Investigação em Epidemiologia (EPIUnit), Instituto de Saúde Pública, University of Porto, Porto, Portugal 5Escola Superior de Saúde, Instituto Politécnico de Leiria, Campus 2 - Morro de Lena - Alto do Vieiro, Leiria, Portugal 6Clinical Pathology Department of São João Hospital Centre, Porto, Portugal 7CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal 8Instituto de Investigação e Inovação em Saúde (i3 s), University of Porto, Porto, Portugal 9Department of Experimental Biology, Faculty of Medicine, University of Porto, Porto, Portugal.

出版信息

Menopause. 2017 May;24(5):563-573. doi: 10.1097/GME.0000000000000780.

DOI:10.1097/GME.0000000000000780

PMID:27898652

Abstract

OBJECTIVE

Prevention or induction of metabolic disorders and obesity depend on estrogen signaling and/or exogenous factors, such as mineral content in diet. The protective effects of a Portuguese natural mineral-rich water against the induction of metabolic syndrome in fructose-fed male Sprague-Dawley rats have been reported. The present study was designed to assess the impact of this mineral-rich water on fructose-fed estrogen-deficient female Sprague-Dawley rats.

METHODS

Ovariectomized rats had access to tap (TWO) or mineral-rich (MWO) waters, with and without 10% fructose (10-wk treatment). A sham-operated (tap water supplied) group was included and each of the five groups included six rats. Plasma biochemical and metabolic parameters were evaluated by routine clinical measurements. Western blotting was used to assess hepatic protein expression of sirtuins (Sirt) 1 and 3, phosphorylated AMP-activated protein kinase-α (p-AMPKα), peroxisome proliferator-activated receptor gamma coactivator-1-α (PGC1α), glucocorticoid receptor, and 11beta-hydroxysteroid dehydrogenase type 1 (11βHSD1).

RESULTS

Ovariectomy increased plasma total cholesterol (46%/P < 0.05), but had no significant effects on hepatic protein expression. Fructose intake by ovariectomized rats increased PGC1α and 11βHSD1 (fructose in tap water [TWFO] vs TWO: 65%/P < 0.05 and 38%/P = 0.05, respectively) as well as glucocorticoid receptor (TWFO and fructose in natural mineral-rich water [MWFO] vs TWO and MWO: 107%/P = 0.05 and 182%/P < 0.05, respectively). Mineral-rich water ingestion exerted an increasing shape on Sirt1 (MWO vs TWO: 76%/P < 0.05; MWFO vs TWFO: 76%/P = 0.06), PGC1α (MWO vs TWO: 77%/P < 0.01), p-AMPKα (MWO vs TWO: 152%/P = 0.01; MWFO vs TWFO: 107%/P = 0.01), and 11βHSD1 (MWO vs TWO: 91%/P = 0.05; MWFO vs TWFO: 47%/P = 0.05).

CONCLUSIONS

Mineral-rich water ingestion may have a prime role on the activation of Sirt1 signaling and the modulation of glucocorticoid signaling in the postmenopause.

摘要

目的

代谢紊乱和肥胖的预防或诱发取决于雌激素信号传导和/或外部因素，如饮食中的矿物质含量。据报道，葡萄牙一种富含天然矿物质的水对用果糖喂养的雄性斯普拉格-道利大鼠诱发代谢综合征具有保护作用。本研究旨在评估这种富含矿物质的水对用果糖喂养的雌激素缺乏雌性斯普拉格-道利大鼠的影响。

方法

去卵巢大鼠可饮用自来水（TWO）或富含矿物质的水（MWO），分别添加和不添加10%果糖（为期10周的处理）。纳入假手术组（供应自来水），五组中的每组包含六只大鼠。通过常规临床测量评估血浆生化和代谢参数。采用蛋白质免疫印迹法评估肝脏中沉默调节蛋白（Sirt）1和3、磷酸化的AMP激活蛋白激酶-α（p-AMPKα）、过氧化物酶体增殖物激活受体γ共激活因子-1-α（PGC1α）、糖皮质激素受体和11β-羟基类固醇脱氢酶1型（11βHSD1）的蛋白表达。

结果

去卵巢使血浆总胆固醇升高（46%/P<0.05），但对肝脏蛋白表达无显著影响。去卵巢大鼠摄入果糖会增加PGC1α和11βHSD1（自来水添加果糖组[TWFO]与饮用自来水组[TWO]相比：分别为65%/P<0.05和38%/P = 0.05）以及糖皮质激素受体（TWFO和天然富含矿物质的水添加果糖组[MWFO]与TWO和MWO相比：分别为107%/P = 0.05和182%/P<0.05）。摄入富含矿物质的水会使Sirt1（MWO与TWO相比：76%/P<0.05；MWFO与TWFO相比：76%/P = 0.06）、PGC1α（MWO与TWO相比：77%/P<0.01）、p-AMPKα（MWO与TWO相比：152%/P = 0.01；MWFO与TWFO相比：107%/P = 0.01）和11βHSD1（MWO与TWO相比：91%/P = 0.05；MWFO与TWFO相比：47%/P = 0.05）增加。