Connor A M, Waterhouse P, Khokha R, Denhardt D T
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Biochim Biophys Acta. 1989 Sep 21;1009(1):75-82. doi: 10.1016/0167-4781(89)90081-x.
A genomic clone containing the complete gene, designated mrp/plf3, of a member of the mitogen-regulated protein/proliferin (MRP/PLF) multi-gene family was isolated from a mouse genomic library constructed in lambda L47.1. The DNA sequences of the 5' flanking region, the exons, and the exon/intron boundaries have been determined. The size and organization of the five exons in the murine mrp/plf3 gene are very similar to those of members of the prolactin/growth hormone family, confirming that mrp/plf3 is a part of this superfamily. Removal of an upstream negative regulatory element revealed an active mrp/plf promoter that was responsive to TGF-alpha and contains known regulatory elements. Intracellular mrp/plf mRNA levels were increased by 17 beta-estradiol and reduced by dexamethasone.
从构建于λ L47.1的小鼠基因组文库中分离出一个基因组克隆，其包含有丝分裂原调节蛋白/增殖素（MRP/PLF）多基因家族成员的完整基因，命名为mrp/plf3。已确定了其5'侧翼区、外显子以及外显子/内含子边界的DNA序列。小鼠mrp/plf3基因中五个外显子的大小和组织与催乳素/生长激素家族成员非常相似，证实mrp/plf3是该超家族的一部分。去除上游负调控元件后发现一个对TGF-α有反应且含有已知调控元件的活性mrp/plf启动子。细胞内mrp/plf mRNA水平在17β-雌二醇作用下升高，在地塞米松作用下降低。
