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编码小鼠有丝分裂原调节蛋白的cDNA克隆的鉴定：永生和永生化细胞系中mRNA水平的调节

Characterization of a cDNA clone encoding murine mitogen-regulated protein: regulation of mRNA levels in mortal and immortal cell lines.

作者信息

Parfett C L, Hamilton R T, Howell B W, Edwards D R, Nilsen-Hamilton M, Denhardt D T

出版信息

Mol Cell Biol. 1985 Nov;5(11):3289-92. doi: 10.1128/mcb.5.11.3289-3292.1985.

DOI:10.1128/mcb.5.11.3289-3292.1985
PMID:3841805
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369146/
Abstract

Mitogen-regulated protein (MRP) is secreted by certain immortal murine cell lines (Swiss 3T3, BNL) stimulated with serum or particular growth factors. We have identified a cDNA clone that encodes part of the protein and have confirmed that MRP is closely related to, if not identical to, the prolactin-related protein designated proliferin. MRP is not produced by primary mouse embryo fibroblasts to nearly the same extent as it is produced by many immortal or transformed lines. Control of expression of this protein by growth factors is achieved both by regulating the extent of transcription and by regulating the processing of the protein.

摘要

丝裂原调节蛋白（MRP）由某些用血清或特定生长因子刺激的永生小鼠细胞系（瑞士3T3、BNL）分泌。我们鉴定出一个编码该蛋白部分序列的cDNA克隆，并证实MRP即使与催乳素相关蛋白增殖素不完全相同，也与之密切相关。原代小鼠胚胎成纤维细胞产生MRP的程度远不及许多永生或转化细胞系。生长因子对该蛋白表达的调控是通过调节转录程度和蛋白加工过程来实现的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/c086bd0267a0/molcellb00141-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/7152739140d0/molcellb00141-0422-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/7e108877ea45/molcellb00141-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/c086bd0267a0/molcellb00141-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/7152739140d0/molcellb00141-0422-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/7e108877ea45/molcellb00141-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/369146/c086bd0267a0/molcellb00141-0423-b.jpg

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1
Characterization of a cDNA clone encoding murine mitogen-regulated protein: regulation of mRNA levels in mortal and immortal cell lines.编码小鼠有丝分裂原调节蛋白的cDNA克隆的鉴定：永生和永生化细胞系中mRNA水平的调节
Mol Cell Biol. 1985 Nov;5(11):3289-92. doi: 10.1128/mcb.5.11.3289-3292.1985.
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Control of proliferin gene expression in serum-stimulated mouse cells.血清刺激的小鼠细胞中增殖蛋白基因表达的调控
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Evidence that post-transcriptional changes in the expression of mitogen regulated protein accompany immortalization of mouse cells.有证据表明，有丝分裂原调节蛋白表达的转录后变化与小鼠细胞永生化相伴发生。
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The mitogen-regulated protein/proliferin transcript is degraded in primary mouse embryo fibroblast but not 3T3 nuclei: altered RNA processing correlates with immortalization.丝裂原调节蛋白/增殖蛋白转录本在原代小鼠胚胎成纤维细胞核中会被降解，但在3T3细胞核中不会：RNA加工过程的改变与永生化相关。
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Identification of proliferin mRNA and protein in mouse placenta.小鼠胎盘中增殖蛋白mRNA和蛋白的鉴定。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4356-9. doi: 10.1073/pnas.82.13.4356.
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Instability of endogenous MRP/proliferin transcripts in the nucleus of mouse embryo fibroblasts contrasts with their stability when produced during transient transfections.小鼠胚胎成纤维细胞核内源性MRP/增殖蛋白转录本的不稳定性与其在瞬时转染过程中产生时的稳定性形成对比。
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Regulation of the expression of mitogen-regulated protein (MRP; proliferin) and cathepsin L in cultured cells and in the murine placenta.
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The mitogen-regulated protein/proliferin transcript is degraded in primary mouse embryo fibroblast but not 3T3 nuclei: altered RNA processing correlates with immortalization.丝裂原调节蛋白/增殖蛋白转录本在原代小鼠胚胎成纤维细胞核中会被降解，但在3T3细胞核中不会：RNA加工过程的改变与永生化相关。
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Synthesis of ds-cDNA involving addition of dCMP tails to allow cloning of 5'-terminal m RNA sequences.双链互补DNA的合成，包括添加dCMP尾以便克隆5'-末端mRNA序列。
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