Lotem J, Shabo Y, Sachs L
Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.
Blood. 1989 Oct;74(5):1545-51.
Megakaryocytes develop in densely seeded normal mouse bone marrow (BM) cells cultured in agar or in liquid medium. This formation of megakaryocytes is enhanced by the myeloid differentiation-inducing protein MGI-2, which we have shown to be interleukin-6 (IL-6). Monoclonal antibody (MoAb) that specifically neutralizes mouse IL-6 but not human IL-6 inhibited megakaryocyte development in cells cultured either with or without the addition of mouse IL-6 but did not inhibit megakaryocyte development induced by human IL-6. This MoAb to mouse IL-6 that does not neutralize mouse IL-3 also inhibited mouse IL-3-induced megakaryocyte development. Antibody to mouse GM-CSF did not inhibit the formation of megakaryocytes. The results show that the induction of megakaryocyte development by IL-3 is due to the production of IL-6 in the BM cultures. The present experiments demonstrate a new property of IL-6 and indicate that IL-6 is a regulatory protein of normal megakaryocyte development.
巨核细胞在接种密度高的正常小鼠骨髓（BM）细胞于琼脂或液体培养基中培养时发育。髓系分化诱导蛋白MGI - 2可增强巨核细胞的这种形成，我们已证明MGI - 2是白细胞介素 - 6（IL - 6）。特异性中和小鼠IL - 6而非人IL - 6的单克隆抗体（MoAb），在添加或不添加小鼠IL - 6培养的细胞中均抑制巨核细胞发育，但不抑制人IL - 6诱导的巨核细胞发育。这种不中和小鼠IL - 3的抗小鼠IL - 6单克隆抗体也抑制小鼠IL - 3诱导的巨核细胞发育。抗小鼠GM - CSF抗体不抑制巨核细胞的形成。结果表明，IL - 3诱导巨核细胞发育是由于BM培养物中IL - 6的产生。本实验证明了IL - 6的一种新特性，并表明IL - 6是正常巨核细胞发育的调节蛋白。
