Nakayama K
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Ann Hematol. 1994 Nov;69(5):245-7. doi: 10.1007/BF01700278.
An autocrine loop in a megakaryocytic cell line, CMK, has been proposed by Fuse et al. They detected IL-6 mRNA by means of Northern blotting, but not IL-6 receptor (IL-6R) mRNA. I also failed to detect IL-6R mRNA by the same means. In this study I showed the expression of IL-6 and its receptor mRNAs by means of reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) with an automated electrophoresis PhastSystem. These two procedures considerably enhanced detection sensitivity. The proliferation and the differentiation of CMK cells are augmented by IL-6 and inhibited by anti-IL-6 antibody. This study continued the autocrine theory of Fuse et al. in CMK cells. Moreover, this is the first detection of IL-6R mRNA in CMK.
古濑等人提出巨核细胞系CMK中存在自分泌环。他们通过Northern印迹法检测到了IL-6 mRNA，但未检测到IL-6受体（IL-6R）mRNA。我采用同样的方法也未能检测到IL-6R mRNA。在本研究中，我通过使用自动电泳PhastSystem的逆转录巢式聚合酶链反应（RT-巢式PCR）显示了IL-6及其受体mRNA的表达。这两种方法大大提高了检测灵敏度。IL-6可增强CMK细胞的增殖和分化，而抗IL-6抗体则可抑制其增殖和分化。本研究延续了古濑等人在CMK细胞中的自分泌理论。此外，这是首次在CMK中检测到IL-6R mRNA。
