Yang M, Chesterman C N, Chong B H
Department of Haematology, School of Pathology, University of New South Wales, Sydney, Australia.
Br J Haematol. 1995 Oct;91(2):285-9. doi: 10.1111/j.1365-2141.1995.tb05291.x.
The effect of recombinant platelet-derived growth factor (PDGF) on both murine and human megakaryocyte colony formation was studied in the plasma clot culture system. PDGF significantly stimulates megakaryocyte colony formation in a dose-dependent manner. The minimum concentration which had a significant stimulating effect on colony forming unit megakaryocyte (CFU-MK) was 10 ng/ml and maximum stimulation occurred at 50 ng/ml. The effect of PDGF was compared with that of interleukin (IL)-3, IL-6, granulocyte-macrophage colony stimulating factor (GM-CSF), erythropoietin (EPO) and acid fibroblast growth factor (aFGF) on megakaryocyte colony formation. The results showed that megakaryocyte colony stimulating activity of PDGF was slightly higher than those of GM-CSF and aFGF, but lower than those of IL-3, IL-6 and EPO. The effect of PDGF in combination with IL-3 or IL-6 on megakaryocyte colony formation was also investigated. No synergistic action was found between PDGF and IL-3 or IL-6, but an additive effect was observed with IL-3 plus IL-6. We also studied the effects of PDGF in combination with anti-IL6, anti-IL-3 or anti-GM-CSF antibody. The increase of megakaryocyte colony formation induced by PDGF was partially inhibited by anti-IL-6 or anti-GM-CSF antibody but not by anti-IL-3 antibody. These results indicate that PDGF is a positive regulator for megakaryocytopoiesis in vitro and IL-6 and GM-CSF may play a role in the mechanism whereby PDGF stimulates megakaryocytopoiesis.
在血浆凝块培养系统中研究了重组血小板衍生生长因子（PDGF）对小鼠和人巨核细胞集落形成的影响。PDGF以剂量依赖方式显著刺激巨核细胞集落形成。对巨核细胞集落形成单位（CFU-MK）有显著刺激作用的最低浓度为10 ng/ml，最大刺激作用出现在50 ng/ml。将PDGF的作用与白细胞介素（IL）-3、IL-6、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、促红细胞生成素（EPO）和酸性成纤维细胞生长因子（aFGF）对巨核细胞集落形成的作用进行了比较。结果表明，PDGF的巨核细胞集落刺激活性略高于GM-CSF和aFGF，但低于IL-3、IL-6和EPO。还研究了PDGF与IL-3或IL-6联合对巨核细胞集落形成的影响。未发现PDGF与IL-3或IL-6之间有协同作用，但观察到IL-3加IL-6有相加作用。我们还研究了PDGF与抗IL-6、抗IL-3或抗GM-CSF抗体联合的作用。抗IL-6或抗GM-CSF抗体部分抑制了PDGF诱导的巨核细胞集落形成增加，但抗IL-3抗体没有。这些结果表明，PDGF是体外巨核细胞生成的正调节因子，IL-6和GM-CSF可能在PDGF刺激巨核细胞生成的机制中起作用。
