Techo Sujitra, Miyashita Mika, Shibata Chiyo, Tanaka Naoto, Wisetkhan Preeyarach, Visessanguan Wonnop, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5500-5505. doi: 10.1099/ijsem.0.001547. Epub 2016 Oct 11.
A Gram-stain-positive, lactic acid bacterium, strain Ru20-1T, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1T belonged to the genus Lactobacillus. The strain was heterofermentative, non-spore-forming and rod-shaped. It produced dl-lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to Lactobacillus lindneri LMG 14528T (96.8 %), Lactobacillus sanfranciscensis NRIC 1548T (95.4 %) and Lactobacillus florum NRIC 0771T (95.2 %), respectively. In addition, the pheS gene sequence of strain Ru20-1T was closely related to those of L. sanfranciscensis NRIC 1548T (92.0 %), L. lindneri LMG 14528T (89.0 %) and L. florum NRIC 0771T(85.0 %). Phylogenetic analysis indicated that strain Ru20-1T was clearly separated from closely related species of the genus Lactobacillus. The DNA G+C content of strain Ru20-1T was 47.8 mol %. The cell-wall peptidoglycan type was l-Lys-d-Asp. The major cellular fatty acids were C18 : 1ω9c, C20 : 0, C20 : 1ω9c and summed feature 7 (unknown 18.846 and/or C19 : 1ω6c and/or C19 : 0 cyclo). On the basis of the data provided, strain Ru20-1T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus ixorae sp. nov. is proposed. The type strain is Ru20-1T (=LMG 29008T=NBRC 111239T=PCU 346T=TISTR 2381T).
从泰国呵叻府采集的一种花（西印度茉莉）中分离出一株革兰氏阳性乳酸菌，菌株Ru20 - 1T。采用多相分类法确定该菌株的分类地位。形态学和生化特性研究表明，菌株Ru20 - 1T属于乳杆菌属。该菌株为异型发酵，不产芽孢，呈杆状。它产生dl - 乳酸。基于16S rRNA基因序列相似性，该菌株分别与林氏乳杆菌LMG 14528T（96.8%）、旧金山乳杆菌NRIC 1548T（95.4%）和弗洛乳杆菌NRIC 0771T（95.2%）密切相关。此外，菌株Ru20 - 1T的pheS基因序列分别与旧金山乳杆菌NRIC 1548T（92.0%）、林氏乳杆菌LMG 14528T（89.0%）和弗洛乳杆菌NRIC 0771T（85.0%）密切相关。系统发育分析表明，菌株Ru20 - 1T与乳杆菌属的近缘物种明显分开。菌株Ru20 - 1T的DNA G + C含量为47.8 mol%。细胞壁肽聚糖类型为l - Lys - d - Asp。主要细胞脂肪酸为C18 : 1ω9c、C20 : 0、C20 : 1ω9c和总和特征7（未知18.846和/或C19 : 1ω6c和/或C19 : 0环）。根据所提供的数据，菌株Ru20 - 1T代表乳杆菌属的一个新物种，为此提出新名称Ixorae乳杆菌（Lactobacillus ixorae sp. nov.）。模式菌株为Ru20 - 1T（=LMG 29008T=NBRC 111239T=PCU 346T=TISTR 2381T）。
